IL2RA monoclonal antibody, clone MEM-181 (FITC)
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More Files
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Flow Cytometry
Surface staining of human PBMC with IL2RA monoclonal antibody, clone MEM-181 (FITC) (Cat # MAB4582).
The mononuclear cells were isolated from human peripheral blood, devided in aliquots for duplicate analysis and stimulated with PHA for 2 days.
Panel A, C : staining with the IL2RA monoclonal antibody, clone MEM-181 (Cat # MAB0882).
Panel B, D : staining with the standard IL2RA monoclonal antibody.
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Specification
Product Description
Mouse monoclonal antibody raised against native IL2RA.
Immunogen
Native purified IL2RA from PHA-activated peripheral blood leucocytes.
Host
Mouse
Theoretical MW (kDa)
55
Reactivity
Human
Specificity
This antibody reacts with CD25 (Interleukin-2 receptor alpha chain), a 55 KDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.
Form
Liquid
Conjugation
FITC
Isotype
IgG1
Recommend Usage
Flow Cytometry (20 ul in human blood cells 100 ul in whole blood or 106 cells in a suspension)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.2% BSA, 0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Flow Cytometry
Surface staining of human PBMC with IL2RA monoclonal antibody, clone MEM-181 (FITC) (Cat # MAB4582).
The mononuclear cells were isolated from human peripheral blood, devided in aliquots for duplicate analysis and stimulated with PHA for 2 days.
Panel A, C : staining with the IL2RA monoclonal antibody, clone MEM-181 (Cat # MAB0882).
Panel B, D : staining with the standard IL2RA monoclonal antibody. -
Gene Info — IL2RA
Entrez GeneID
3559Gene Name
IL2RA
Gene Alias
CD25, IDDM10, IL2R, TCGFR
Gene Description
interleukin 2 receptor, alpha
Gene Ontology
HyperlinkGene Summary
The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. [provided by RefSeq
Other Designations
Interleukin-2 receptor|OTTHUMP00000019030|OTTHUMP00000044870|interleukin 2 receptor, alpha chain
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Interactome
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Pathway
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Disease
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Publication Reference
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CD25 indicates the neoplastic phenotype of mast cells: a novel immunohistochemical marker for the diagnosis of systemic mastocytosis (SM) in routinely processed bone marrow biopsy specimens.
Sotlar K, Horny HP, Simonitsch I, Krokowski M, Aichberger KJ, Mayerhofer M, Printz D, Fritsch G, Valent P.
The American Journal of Surgical Pathology 2004 Oct; 28(10):1319.
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Advances in interleukin 2 receptor targeted treatment.
Morris JC, Waldmann TA.
Annals of the Rheumatic Diseases 2000 Nov; 59:i109.
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The interleukin-2 receptor in human monocytes and macrophages: regulation of expression and release of the alpha and beta chains (p55 and p75).
C Scheibenbogen, U Keilholz, M Richter, R Andreesen, W Hunstein.
Research in Immunology 1992 Jan; 143(1):33.
Application：Flow Cyt, Human, Human macrophages, Human monocytes .
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CD25 indicates the neoplastic phenotype of mast cells: a novel immunohistochemical marker for the diagnosis of systemic mastocytosis (SM) in routinely processed bone marrow biopsy specimens.
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