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Last updated: 2016/10/23
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IL2RA monoclonal antibody, clone MEM-181 (FITC)

  • Catalog # : MAB4582
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against native IL2RA.
  • Immunogen:
  • Native purified IL2RA from PHA-activated peripheral blood leucocytes.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 55
  • Reactivity:
  • Human
  • Specificity:
  • This antibody reacts with CD25 (Interleukin-2 receptor alpha chain), a 55 KDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.
  • Form:
  • Liquid
  • Conjugation:
  • FITC
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Flow Cytometry (20 ul in human blood cells 100 ul in whole blood or 106 cells in a suspension)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.2% BSA, 0.09% sodium azide)
  • Storage Instruction:
  • Store in the dark at 4°C. Do not freeze.
    Avoid prolonged exposure to light.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Flow Cytometry
  • Flow Cytometry
  • Surface staining of human PBMC with IL2RA monoclonal antibody, clone MEM-181 (FITC) (Cat # MAB4582).
    The mononuclear cells were isolated from human peripheral blood, devided in aliquots for duplicate analysis and stimulated with PHA for 2 days.
    Panel A, C : staining with the IL2RA monoclonal antibody, clone MEM-181 (Cat # MAB0882).
    Panel B, D : staining with the standard IL2RA monoclonal antibody.
  • Application Image
  • Gene Information
  • Entrez GeneID:
  • 3559
  • Gene Name:
  • IL2RA
  • Gene Alias:
  • Gene Description:
  • interleukin 2 receptor, alpha
  • Gene Summary:
  • The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. [provided by RefSeq
  • Other Designations:
  • Interleukin-2 receptor,OTTHUMP00000019030,OTTHUMP00000044870,interleukin 2 receptor, alpha chain
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