Mouse monoclonal antibody raised against native IL2RA.
Native purified IL2RA from PHA-activated peripheral blood leucocytes.
Theoretical MW (kDa):
This antibody reacts with CD25 (Interleukin-2 receptor alpha chain), a 55 KDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.
Flow Cytometry (20 ul in human blood cells 100 ul in whole blood or 106 cells in a suspension) The optimal working dilution should be determined by the end user.
In PBS (0.2% BSA, 0.09% sodium azide)
Store in the dark at 4°C. Do not freeze. Avoid prolonged exposure to light. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Surface staining of human PBMC with IL2RA monoclonal antibody, clone MEM-181 (FITC) (Cat # MAB4582). The mononuclear cells were isolated from human peripheral blood, devided in aliquots for duplicate analysis and stimulated with PHA for 2 days. Panel A, C : staining with the IL2RA monoclonal antibody, clone MEM-181 (Cat # MAB0882). Panel B, D : staining with the standard IL2RA monoclonal antibody.
The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. [provided by RefSeq