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Last updated: 2017/7/23
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TP53 monoclonal antibody, clone BP53-12 (FITC)

  • Catalog # : MAB4528
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant TP53.
  • Immunogen:
  • Recombinant protein corresponding to full length human TP53.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 50
  • Reactivity:
  • Human, Non-Human Primates
  • Specificity:
  • This antibody recognizes defined epitope (aa 16-25) on human p53, a 50 KDa tumour suppressor found in increased amounts in a wide variety of transformed cells.
  • Form:
  • Liquid
  • Conjugation:
  • FITC
  • Concentration:
  • 1 mg/mL
  • Isotype:
  • IgG2a
  • Recommend Usage:
  • The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.2 (0.09% sodium azide)
  • Storage Instruction:
  • Store in the dark at 4°C. Do not freeze.
    Avoid prolonged exposure to light.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Immunofluorescence
  • Immunofluorescence
  • Confocal microscopy of human HeLa cells using TP53 monoclonal antibody, clone BP53-12 (FITC) (Cat # MAB4528). The expression of TP53 protein was enhanced by intercalating reagent.
    Cells were fixed and permeabilized before incubation with the TP53 monoclonal antibody, clone BP53-12 (FITC) (Cat # MAB4528).
    Photo provided by Dr. Hodny, Inst. of Experimental Medicine, Prague, Czech Republic.
  • Flow Cytometry
  • Application Image
  • Flow Cytometry
  • Gene Information
  • Entrez GeneID:
  • 7157
  • Gene Name:
  • TP53
  • Gene Alias:
  • FLJ92943,LFS1,TRP53,p53
  • Gene Description:
  • tumor protein p53
  • Gene Summary:
  • This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity. [provided by RefSeq
  • Other Designations:
  • p53 antigen,p53 transformation suppressor,p53 tumor suppressor,phosphoprotein p53,transformation-related protein 53
  • Interactome
  • Interactome
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