VIM monoclonal antibody, clone VI-RE/1 (PE)
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More Files
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Flow Cytometry
Intracellular flow cytometry analysis of VIM expression in LEP-19 (human fibroblast cell line) using VIM monoclonal antibody, clone VI-RE/1 (PE) (Cat # MAB4527).
Overlay with Isotype mouse IgG1 control.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant VIM.
Immunogen
Recombinant protein corresponding to human VIM.
Host
Mouse
Theoretical MW (kDa)
57
Reactivity
Human
Specificity
This antibody reacts with human vimentin, a 57 KDa intermediate filament protein expressed on a wide variety of mesenchymal and mesodermal cell types.
Form
Liquid
Conjugation
PE
Concentration
0.1 mg/mL
Isotype
IgG1
Recommend Usage
Flow Cytometry (10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.2% BSA, 0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunocytochemistry
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Intracellular flow cytometry analysis of VIM expression in LEP-19 (human fibroblast cell line) using VIM monoclonal antibody, clone VI-RE/1 (PE) (Cat # MAB4527).
Overlay with Isotype mouse IgG1 control. -
Gene Info — VIM
Entrez GeneID
7431Gene Name
VIM
Gene Alias
FLJ36605
Gene Description
vimentin
Omim ID
193060Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract
Other Designations
OTTHUMP00000019224
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Interactome
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Disease
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Publication Reference
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Metastatic ability and the epithelial-mesenchymal transition in induced cancer stem-like hepatoma cells.
Nishiyama M, Tsunedomi R, Yoshimura K, Hashimoto N, Matsukuma S, Ogihara H, Kanekiyo S, Iida M, Sakamoto K, Suzuki N, Takeda S, Yamamoto S, Yoshino S, Ueno T, Hamamoto Y, Hazama S, Nagano H.
Cancer Science 2018 Apr; 109(4):1101.
Application：Flow Cyt, Human, SK-HEP-1 cells.
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Metastatic ability and the epithelial-mesenchymal transition in induced cancer stem-like hepatoma cells.
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