HLA-G monoclonal antibody, clone MEM-G/11 (FITC)
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More Files
- More Functions
Flow Cytometry
Surface staining of JEG-3 (human choriocarcinoma cell line) with HLA-G monoclonal antibody, clone MEM-G/11 (FITC) (Cat # MAB4520).
Total viable cells were used for analysis.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant HLA-G.
Immunogen
Recombinant protein corresponding to human HLA-G.
Host
Mouse
Reactivity
Human
Specificity
This antibody recognizes HLA-G1 antigen. HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.
Form
Liquid
Conjugation
FITC
Concentration
1 mg/mL
Isotype
IgG1
Recommend Usage
Flow Cytometry (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Surface staining of JEG-3 (human choriocarcinoma cell line) with HLA-G monoclonal antibody, clone MEM-G/11 (FITC) (Cat # MAB4520).
Total viable cells were used for analysis. -
Gene Info — HLA-G
Entrez GeneID
3135Gene Name
HLA-G
Gene Alias
MHC-G
Gene Description
major histocompatibility complex, class I, G
Gene Ontology
HyperlinkGene Summary
HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq
Other Designations
HLA class I molecule|HLA-G histocompatibility antigen, class I, G|MHC class I antigen|OTTHUMP00000039485|b2 microglobulin
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Interactome
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Pathway
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Disease
- Abortion
- Anemia
- Arthritis
- Asthma
- Autoimmune Diseases
- Behcet Syndrome
- Birth Weight
- Bronchial Hyperreactivity
- Carcinoma
+ View More Disease
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Publication Reference
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Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules.
Menier C, Saez B, Horejsi V, Martinozzi S, Krawice-Radanne I, Bruel S, Le Danff C, Reboul M, Hilgert I, Rabreau M, Larrad ML, Pla M, Carosella ED, Rouas-Freiss N.
Human Immunology 2003 Mar; 64(3):315.
Application：Flow Cyt, Human, LCL, JEG-3, K-563 cells.
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Disulfide bond-mediated dimerization of HLA-G on the cell surface.
Boyson JE, Erskine R, Whitman MC, Chiu M, Lau JM, Koopman LA, Valter MM, Angelisova P, Horejsi V, Strominger JL.
PNAS 2002 Dec; 99(25):16180.
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Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules.
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