Myc monoclonal antibody, clone 9E10 (HRP)
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of Myc.
Immunogen
A synthetic peptide corresponding to human MYC.
Sequence
AEEQKLISEEDLL
Host
Mouse
Reactivity
Human
Specificity
This antibody may be used to detect the c-Myc tag.
Form
Liquid
Conjugation
HRP
Concentration
1 mg/mL
Isotype
IgG1
Recommend Usage
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (1% BSA, 0.01% thimerosal)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains thimerosal: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoprecipitation
Flow Cytometry
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Publication Reference
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The Csk-binding protein PAG regulates PDGF-induced Src mitogenic signaling via GM1.
Veracini L, Simon V, Richard V, Schraven B, Horejsi V, Roche S, Benistant C.
The Journal of Cell Biology 2008 Aug; 182(3):603.
Application：WB-Tr, Mouse, MEF, NIH/3T3 cells.
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Enhancement of scFv fragment reactivity with target antigens in binding assays following mixing with anti-tag monoclonal antibodies.
Wang X, Campoli M, Ko E, Luo W, Ferrone S.
Journal of Immunology 2004 Nov; 294(1-2):23.
Application：ELISA, Flow Cyt, Human, Recombinant protein.
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A single-chain antibody/epitope system for functional analysis of protein-protein interactions.
Fujiwara K, Poikonen K, Aleman L, Valtavaara M, Saksela K, Mayer BJ.
Biochemistry 2002 Oct; 41(42):12729.
Application：WB-Tr, Human, HEK 293T cells.
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The Csk-binding protein PAG regulates PDGF-induced Src mitogenic signaling via GM1.
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