Flow Cytometry analysis of basophil activation upon stimulation of normal (heparin-treated) whole blood with combination of IL-3 and Goat anti-IgE polyclonal antibody. Combination of IgE monoclonal antibody, clone BE5 (FITC) (Cat # MAB4491) and IgE monoclonal antibody, clone BE5 (PE) (Cat # MAB4492)-Dyomics 647 was used (analysis in basophil wIDO1w). Fig. A - staining of non-stimulated (control) sample. Fig. B - staining of IgE stimulated sample.