IgE monoclonal antibody, clone BE5 (PE)
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More Files
- More Functions
Flow Cytometry
Flow Cytometry analysis of basophil activation upon stimulation of normal (heparin-treated) whole blood with combination of IL-3 and Goat anti-IgE polyclonal antibody.
Combination of IgE monoclonal antibody, clone BE5 (FITC) (Cat # MAB4491) and IgE monoclonal antibody, clone BE5 (PE) (Cat # MAB4492)-Dyomics 647 was used (analysis in basophil wIDO1w).
Fig. A - staining of non-stimulated (control) sample.
Fig. B - staining of IgE stimulated sample.
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Specification
Product Description
Mouse monoclonal antibody raised against IgE.
Immunogen
Human IgE.
Host
Mouse
Reactivity
Human
Specificity
This antibody reacts with human IgE; it recognizes an epitope different from the ones recognized by MAB6451 and MAB6452 antibodies to IgE.
Form
Liquid
Conjugation
PE
Concentration
0.1 mg/mL
Isotype
IgG
Recommend Usage
Flow Cytometry (5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.2% BSA, 0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow Cytometry analysis of basophil activation upon stimulation of normal (heparin-treated) whole blood with combination of IL-3 and Goat anti-IgE polyclonal antibody.
Combination of IgE monoclonal antibody, clone BE5 (FITC) (Cat # MAB4491) and IgE monoclonal antibody, clone BE5 (PE) (Cat # MAB4492)-Dyomics 647 was used (analysis in basophil wIDO1w).
Fig. A - staining of non-stimulated (control) sample.
Fig. B - staining of IgE stimulated sample. -
Publication Reference
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IgE isotype determination: epsilon-germline gene transcription, DNA recombination and B-cell differentiation.
H J Gould, R L Beavil, D Vercelli.
British Medical Bulletin 2000 Jan; 56(4):908.
Application：Flow Cyt, Human, Human B cells.
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T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors.
Brinkmann V, Heusser CH.
Cellular Immunology 1993 Dec; 152(2):323.
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Serological abnormalities in juvenile chronic arthritis: a review of 46 cases.
Balogh Z, Meretey K, Falus A, Bozsoky S.
Annals of the Rheumatic Diseases 1980 Apr; 39(2):129.
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IgE isotype determination: epsilon-germline gene transcription, DNA recombination and B-cell differentiation.
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