CD86 monoclonal antibody, clone BU63 (APC)
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Specifications
Product Description
Mouse monoclonal antibody raised against native CD86.
Immunogen
Native purified CD86 from human ARH-77 cell.
Host
Mouse
Theoretical MW (kDa)
70
Reactivity
Human
Specificity
This antibody reacts with CD86 (B7-2), a 70 KDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
Form
Liquid
Conjugation
APC
Isotype
IgG1
Recommend Usage
Flow Cytometry (10 ul in human blood cells 100 ul in whole blood or 106 cells in a suspension)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.2% BSA, 0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Frozen sections)
Immunoprecipitation
Functional Study
Flow Cytometry
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Gene Info — CD86
Entrez GeneID
942Gene Name
CD86
Gene Alias
B7-2, B70, CD28LG2, LAB72, MGC34413
Gene Description
CD86 molecule
Omim ID
601020Gene Ontology
HyperlinkGene Summary
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms. Additional transcript variants have been described, but their full-length sequences have not been determined. [provided by RefSeq
Other Designations
B-lymphocyte activation antigen B7-2|B7-2 antigen|CD28 antigen ligand 2|CD86 antigen|CD86 antigen (CD28 antigen ligand 2, B7-2 antigen)|CTLA-4 counter-receptor B7.2
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Suppression of colon inflammation by CD80 blockade: evaluation in two murine models of inflammatory bowel disease.
Eri R, Kodumudi KN, Summerlin DJ, Srinivasan M.
Inflammatory Bowel Diseases 2008 Apr; 14(4):458.
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Insertion of host-derived costimulatory molecules CD80 (B7.1) and CD86 (B7.2) into human immunodeficiency virus type 1 affects the virus life cycle.
Giguere JF, Bounou S, Paquette JS, Madrenas J, Tremblay MJ.
Journal of Virology 2004 Jun; 78(12):6222.
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The B7-2 (B70) costimulatory molecule expressed by monocytes and activated B lymphocytes is the CD86 differentiation antigen.
Engel P, Gribben JG, Freeman GJ, Zhou LJ, Nozawa Y, Abe M, Nadler LM, Wakasa H, Tedder TF.
Blood 1992 Jun; 69(6):915.
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Suppression of colon inflammation by CD80 blockade: evaluation in two murine models of inflammatory bowel disease.
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