CD52 monoclonal antibody, clone HI186 (APC)
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Specification
Product Description
Mouse monoclonal antibody raised against native CD52.
Immunogen
Native purified CD52 from human tonsil.
Host
Mouse
Reactivity
Human
Specificity
This antibody reacts with CD52 (CAMPATH-1), a 21-28 KDa glycoprotein containing a large N-linked carbohydrate moiety; mature CD52 molecule is actually much smaller (approx. 8-9 KDa). CD52 is expressed at high levels on lymphocytes, monocytes/macrophages and in male reproductive tract.
Form
Liquid
Conjugation
APC
Isotype
IgG2b
Recommend Usage
Flow Cytometry (10 ul in human blood cells 100 ul in whole blood or 106 cells in a suspension)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.2% BSA, 0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Flow Cytometry
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Gene Info — CD52
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Interactome
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Publication Reference
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Role of male reproductive tract CD52 (mrt-CD52) in reproduction.
Koyama K, Ito K, Hasegawa A.
Soc Reprod Fertil Suppl 2007 Jan; 63:103.
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CD52 antigen--a review.
Domagala A, Kurpisz M.
Medical Science Monitor: International Medical Journal of Experimental and Clinical Research 2001 Mar; 7(2):325.
Application：Flow Cyt, IF, WB, Human, Human spermatozoa.
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Male-specific modification of human CD52.
Schröter S, Derr P, Conradt HS, Nimtz M, Hale G, Kirchhoff C.
The Journal of Biological Chemistry 1999 Oct; 274(42):29862.
Application：WB, Human, Lymphocytes, Seminal plasma.
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Role of male reproductive tract CD52 (mrt-CD52) in reproduction.
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