CD177 monoclonal antibody, clone MEM-166 (FITC)
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More Files
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Flow Cytometry
Surface staining of human peripheral blood cells with CD177 monoclonal antibody, clone MEM-166 (FITC) (Cat # MAB4365).
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Specification
Product Description
Mouse monoclonal antibody raised against native CD177.
Immunogen
Native purified CD177 from human granulocytes.
Host
Mouse
Theoretical MW (kDa)
60
Reactivity
Human, Non-Human Primates
Specificity
This antibody reacts with CD177 (Neutrophil specific antigen 1), a 60 KDa GPI-linked cell surface glycoprotein of uPAR family, expressed on granulocytes and in bone marrow early erythroblasts, megakaryocytes, promyelocytes and myelocytes.
Form
Liquid
Conjugation
FITC
Isotype
IgG1
Recommend Usage
Flow Cytometry (20 ul in human blood cells 100 ul in whole blood or 106 cells in a suspension)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.2% BSA, 0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunoprecipitation
Flow Cytometry
Surface staining of human peripheral blood cells with CD177 monoclonal antibody, clone MEM-166 (FITC) (Cat # MAB4365). -
Gene Info — CD177
Entrez GeneID
57126Gene Name
CD177
Gene Alias
HNA2A, NB1, PRV1
Gene Description
CD177 molecule
Omim ID
162860Gene Ontology
HyperlinkGene Summary
NB1, a glycosyl-phosphatidylinositol (GPI)-linked N-glycosylated cell surface glycoprotein, was first described in a case of neonatal alloimmune neutropenia (Lalezari et al., 1971 [PubMed 5552408]).[supplied by OMIM
Other Designations
CD177 antigen|cell surface receptor|polycythemia rubra vera 1
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Interactome
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Publication Reference
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The neutrophil-specific antigen CD177 is a counter-receptor for platelet endothelial cell adhesion molecule-1 (CD31).
Sachs UJ, Andrei-Selmer CL, Maniar A, Weiss T, Paddock C, Orlova VV, Choi EY, Newman PJ, Preissner KT, Chavakis T, Santoso S.
The Journal of Biological Chemistry 2007 Aug; 282(32):23603.
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Proteinase 3 and CD177 are expressed on the plasma membrane of the same subset of neutrophils.
Bauer S, Abdgawad M, Gunnarsson L, Segelmark M, Tapper H, Hellmark T.
Journal of Leukocyte Biology 2007 Feb; 81(2):458.
Application：Flow Cyt, IF, Human, Human neutrophils.
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Expression of polycythemia rubra vera-1 decreases the dependency of cells on growth factors for proliferation.
Mnjoyan Z, Li J, Afshar-Kharghan V.
Haematologica 2005 Mar; 90(3):405.
Application：Flow Cyt, Mouse, CHO-PRV-1 cells.
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The neutrophil-specific antigen CD177 is a counter-receptor for platelet endothelial cell adhesion molecule-1 (CD31).
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