The extracts of mouse brain (50 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with HOMER1 monoclonal antibody, clone AT1F3 (Cat # MAB4202 ; 1 : 1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of U87 MG cells. The cell was stained with HOMER1 monoclonal antibody, clone AT1F3 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
This gene encodes a member of the homer family of dendritic proteins. Members of this family regulate group 1 metabotrophic glutamate receptor function. [provided by RefSeq
Other Designations:
OTTHUMP00000165782,homer 1,homer, neuronal immediate early gene, 1