HOMER1 monoclonal antibody, clone AT1F3
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More Files
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Western Blot (Tissue lysate)
The extracts of mouse brain (50 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with HOMER1 monoclonal antibody, clone AT1F3 (Cat # MAB4202 ; 1 : 1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunofluorescence
Immunofluorescence analysis of U87 MG cells. The cell was stained with HOMER1 monoclonal antibody, clone AT1F3 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant HOMER1.
Immunogen
Recombinant protein corresponding to full length human HOMER1.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Isotype
IgG2b, kappa
Recommend Usage
ELISA
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
The extracts of mouse brain (50 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with HOMER1 monoclonal antibody, clone AT1F3 (Cat # MAB4202 ; 1 : 1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of U87 MG cells. The cell was stained with HOMER1 monoclonal antibody, clone AT1F3 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — HOMER1
Entrez GeneID
9456Protein Accession#
NP_004263Gene Name
HOMER1
Gene Alias
HOMER, HOMER1A, HOMER1B, HOMER1C, SYN47, Ves-1
Gene Description
homer homolog 1 (Drosophila)
Omim ID
604798Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the homer family of dendritic proteins. Members of this family regulate group 1 metabotrophic glutamate receptor function. [provided by RefSeq
Other Designations
OTTHUMP00000165782|homer 1|homer, neuronal immediate early gene, 1
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Interactome
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Disease
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Publication Reference
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The effects of prenatal H1N1 infection at E16 on FMRP, glutamate, GABA, and reelin signaling systems in developing murine cerebellum.
Fatemi SH, Folsom TD, Liesch SB, Kneeland RE, Karkhane Yousefi M, Thuras PD.
Journal of Neuroscience Research 2016 Oct; [Epub].
Application：WB-Ti, Mouse, Brain tissue.
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Homers regulate drug-induced neuroplasticity: implications for addiction.
Szumlinski KK, Ary AW, Lominac KD.
Biochemical Pharmacology 2007 Jul; 75(1):112.
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Homer 1b regulates the trafficking of group I metabotropic glutamate receptors.
Gaddis NC, Chertova E, Sheehy AM, Henderson LE, Malim MH, Roche KW, Tu JC, Petralia RS, Xiao B, Wenthold RJ, Worley PF.
The Journal of Biological Chemistry 1999 Sep; 274(36):25953.
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The effects of prenatal H1N1 infection at E16 on FMRP, glutamate, GABA, and reelin signaling systems in developing murine cerebellum.
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