CD9 monoclonal antibody, clone B3346M
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Specification
Product Description
Mouse monoclonal antibody raised against native CD9.
Immunogen
Native purified CD9 from human bone marrow malignant cells from acute non-T, non-B leukemia.
Host
Mouse
Reactivity
Human
Specificity
The molecular weight of the recognized antigen is 24 KDa. Recognizes 22-43% of peripheral non-T cells (B cells). Recognizes blood platelets and a few granulocytic cells of normal bone marrow. Recognizes the distal convoluted tubule and the distal right tubule of nephrons.
Form
Lyophilized
Isotype
IgG1, kappa
Recommend Usage
Flow Cytometry (2 ug/5x105 cells/test)
Immunofluorescence (2 ug/5x105 cells/test)
Immunohistochemistry (Frozen sections or cell smears) (1:25-1:50)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS (1 mg/mL BSA).
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with 1 mL distilled water for a 0.2 mg/mL concentration, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. The addition of 0.1% (w/v) sodium azide is recommended for storage up to one month at 2-8°C. -
Applications
Immunohistochemistry (Frozen sections)
Immunofluorescence
Flow Cytometry
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Gene Info — CD9
Entrez GeneID
928Gene Name
CD9
Gene Alias
5H9, BA2, BTCC-1, DRAP-27, GIG2, MIC3, MRP-1, P24, TSPAN29
Gene Description
CD9 molecule
Omim ID
143030Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It can modulate cell adhesion and migration and also trigger platelet activation and aggregation. In addition, the protein appears to promote muscle cell fusion and support myotube maintenance. [provided by RefSeq
Other Designations
5H9 antigen|CD9 antigen|CD9 antigen (p24)|OTTHUMP00000041574|OTTHUMP00000041576|antigen defined by monoclonal antibody 602-29|growth-inhibiting gene 2 protein|leukocyte antigen MIC3|motility related protein|motility related protein-1|p24 antigen
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Publication Reference
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Characteristics of platelet aggregation induced by the monoclonal antibody ALB6 (acute lymphoblastic leukemia antigen p 24). Inhibition of aggregation by ALB6Fab.
Boucheix C, Soria C, Mirshahi M, Soria J, Perrot JY, Fournier N, Billard M, Rosenfeld C.
FEBS Letters 1983 Sep; 161(2):289.
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Characteristics of platelet aggregation induced by the monoclonal antibody ALB6 (acute lymphoblastic leukemia antigen p 24). Inhibition of aggregation by ALB6Fab.
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