Mouse monoclonal antibody raised against synthetic peptide of CTNNB1.
A synthetic peptide corresponding to CTNNB1.
Dog, Human, Mouse
Specifically interacts with beta-catenin. The alpha-, beta- and gamma-catenins are cytoplasmic proteins mediating the interaction of Ca2+ -dependent transmembrane adhesion molecules (cadherins) with the cytoskeletal network. The direct interaction of beta-catenin with the cytoplasmic domain of cadherins plays a crucial role for cell-cell adhesion and signal transmission between neighboring cells. Recent studies indicate that beta-catenin may also play a role in tumorigenesis since it forms complexes with the tumor suppressor gene product APC. Beta-catenin directly interacts and constitutively activates transcription factors of the TCF/LEF gene family. Thus it is proposed that beta-catenin plays a dual role not only in the maintenance and regulation of cell-cell interactions but also in the regulation of gene activity. Cross reacts with human, mouse and dog.
ELISA (0.05 ug/mL) Western Blot (0.5 ug/mL for HRP/ECL detection) Immunocytochemistry (1-10 ug/mL) The optimal working dilution should be determined by the end user.
Lyophilized from 2x PBS (PEG, sucrose, 0.09% sodium azide)
Store at 4°C on dry atmosphere. After reconstitution with deionized water, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Detection of endogenous CTNNB1from whole cell lysates of serum starved tumor cells (20,000 cells per lane). The blot was probed with 0.5 ug/mL CTNNB1 monoclonal antibody, clone BDI480 (Cat # MAB3925) for 1 hour at RT and developed by ECL (exp. time : 30 sec). Lane 1 : A-431. Lane 2 : SW480. Lane 3 : SW620. Lane 4 : HT-29. Lane 5 : MCF-7. Lane 6 : MDA-MB-231. Lane 7 : T-47D.