CTNNB1 monoclonal antibody, clone BDI480
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More Files
- More Functions
Western Blot (Cell lysate)
Detection of endogenous CTNNB1from whole cell lysates of serum starved tumor cells (20,000 cells per lane).
The blot was probed with 0.5 ug/mL CTNNB1 monoclonal antibody, clone BDI480 (Cat # MAB3925) for 1 hour at RT and developed by ECL (exp. time : 30 sec).
Lane 1 : A-431.
Lane 2 : SW480.
Lane 3 : SW620.
Lane 4 : HT-29.
Lane 5 : MCF-7.
Lane 6 : MDA-MB-231.
Lane 7 : T-47D.
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of CTNNB1.
Immunogen
A synthetic peptide corresponding to CTNNB1.
Host
Mouse
Reactivity
Dog, Human, Mouse
Specificity
Specifically interacts with beta-catenin. The alpha-, beta- and gamma-catenins are cytoplasmic proteins mediating the interaction of Ca2+ -dependent transmembrane adhesion molecules (cadherins) with the cytoskeletal network. The direct interaction of beta-catenin with the cytoplasmic domain of cadherins plays a crucial role for cell-cell adhesion and signal transmission between neighboring cells. Recent studies indicate that beta-catenin may also play a role in tumorigenesis since it forms complexes with the tumor suppressor gene product APC. Beta-catenin directly interacts and constitutively activates transcription factors of the TCF/LEF gene family. Thus it is proposed that beta-catenin plays a dual role not only in the maintenance and regulation of cell-cell interactions but also in the regulation of gene activity. Cross reacts with human, mouse and dog.
Form
Lyophilized
Isotype
IgG1
Recommend Usage
ELISA (0.05 ug/mL)
Western Blot (0.5 ug/mL for HRP/ECL detection)
Immunocytochemistry (1-10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 2x PBS (PEG, sucrose, 0.09% sodium azide)
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Detection of endogenous CTNNB1from whole cell lysates of serum starved tumor cells (20,000 cells per lane).
The blot was probed with 0.5 ug/mL CTNNB1 monoclonal antibody, clone BDI480 (Cat # MAB3925) for 1 hour at RT and developed by ECL (exp. time : 30 sec).
Lane 1 : A-431.
Lane 2 : SW480.
Lane 3 : SW620.
Lane 4 : HT-29.
Lane 5 : MCF-7.
Lane 6 : MDA-MB-231.
Lane 7 : T-47D.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Enzyme-linked Immunoabsorbent Assay
Enzyme Immunoassay
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