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CTNNB1 monoclonal antibody, clone BDI480

  • Catalog # : MAB3925
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic peptide of CTNNB1.
  • Immunogen:
  • A synthetic peptide corresponding to CTNNB1.
  • Host:
  • Mouse
  • Reactivity:
  • Dog, Human, Mouse
  • Specificity:
  • Specifically interacts with beta-catenin. The alpha-, beta- and gamma-catenins are cytoplasmic proteins mediating the interaction of Ca2+ -dependent transmembrane adhesion molecules (cadherins) with the cytoskeletal network. The direct interaction of beta-catenin with the cytoplasmic domain of cadherins plays a crucial role for cell-cell adhesion and signal transmission between neighboring cells. Recent studies indicate that beta-catenin may also play a role in tumorigenesis since it forms complexes with the tumor suppressor gene product APC. Beta-catenin directly interacts and constitutively activates transcription factors of the TCF/LEF gene family. Thus it is proposed that beta-catenin plays a dual role not only in the maintenance and regulation of cell-cell interactions but also in the regulation of gene activity. Cross reacts with human, mouse and dog.
  • Form:
  • Lyophilized
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (0.05 ug/mL)
    Western Blot (0.5 ug/mL for HRP/ECL detection)
    Immunocytochemistry (1-10 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 2x PBS (PEG, sucrose, 0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C on dry atmosphere.
    After reconstitution with deionized water, store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Detection of endogenous CTNNB1from whole cell lysates of serum starved tumor cells (20,000 cells per lane).
    The blot was probed with 0.5 ug/mL CTNNB1 monoclonal antibody, clone BDI480 (Cat # MAB3925) for 1 hour at RT and developed by ECL (exp. time : 30 sec).
    Lane 1 : A-431.
    Lane 2 : SW480.
    Lane 3 : SW620.
    Lane 4 : HT-29.
    Lane 5 : MCF-7.
    Lane 6 : MDA-MB-231.
    Lane 7 : T-47D.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunocytochemistry
  • Enzyme Immunoassay
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunocytochemistry
  • Enzyme Immunoassay
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