Mouse monoclonal antibody raised against partial recombinant CDH15.
Recombinant protein corresponding to extracellular region of human CDH15.
Cadherins are a family of transmembrane glycoproteins that play a key role in Calcium dependent cell-cell adhesion. Several members of the cadherin family have been identified so far, including E- (epithelial), P- (placental), N- (neuronal) and M- (muscle) cadherin. M-Cadherin is expressed in myoblasts and might be involved in skeletal muscle cell differentiation. Does not cross-react with mouse.
ELISA (0.05 ug/mL) Western Blot (1 ug/mL for AP/BCIP/NBT (MTT) detection 0.5 ug/mL for HRP/ECL detection) Immunofluorescence (5 ug/mL) The optimal working dilution should be determined by the end user.
Lyophilized from 2x PBS (PEG, sucrose, 0.09% sodium azide)
Store at 4°C on dry atmosphere. After reconstitution with deionized water, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of whole cell lysates with CDH15 monoclonal antibody, clone 21G4 (Cat # MAB3922) for 1 hour at RT and developed by ECL. Lane 1 : COS-7. Lane 2 : Human CDH15 overexpressed in COS-7 cells.
This gene is a member of the cadherin superfamily of genes, encoding calcium-dependent intercellular adhesion glycoproteins. Cadherins consist of an extracellular domain containing 5 cadherin domains, a transmembrane region, and a conserved cytoplasmic domain. Transcripts from this particular cadherin are expressed in myoblasts and upregulated in myotubule-forming cells. The protein is thought to be essential for the control of morphogenetic processes, specifically myogenesis, and may provide a trigger for terminal muscle cell differentiation. [provided by RefSeq