VIM monoclonal antibody, clone VI-01
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More Files
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Western Blot (Cell lysate)
Western Blotting analysis of VIM in whole cell lysate of LEP-19 (human fibroblast cell line) (1, 3) and NIH/3T3 (mouse embryonal fibroblast cell line) (2, 4).
Lane 1, 2 : Immunostaining with VIM monoclonal antibody, clone VI-01 (Cat # MAB3880).
Lane 3, 4 : Immunostaining with VIM monoclonal antibody, clone VI-RE/1.
Immunofluorescence
Immunofluorescence staining of RBL (rat basophilic cell line) with VIM monoclonal antibody, clone VI-01 (Cat # MAB3880) conjugated with Dyomics 547. Nuclei are stained with DAPI (blue).
Immunofluorescence
Immunofluorescence staining of NIH/3T3 (mouse embryonal fibroblast cell line) with VIM monoclonal antibody, clone VI-01 (Cat # MAB3880). Nuclei are stained with DAPI (blue).
Immunoprecipitation
Immunoprecipitation of VIM from HeLa cell lysate and detection by VIM monoclonal antibody, clone VI-01 (Cat # MAB3880).
IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated.
MW of VIM is 57 kDa.
Lr = lysate (reducing conditions). Lnr = lysate (non-reducing conditions). IPr = immunoprecipitate (reducing conditions). IPnr = immunoprecipitate (non-reducing conditions).
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Specification
Product Description
Mouse monoclonal antibody raised against native VIM.
Immunogen
Native purified VIM from porcine brain cold stable proteins after depolymerization of microtubules.
Host
Mouse
Theoretical MW (kDa)
57
Reactivity
Mammals, Pig
Specificity
This antibody reacts with vimentin, a 57 KDa intermediate filament expressed in varietyof mesenchymal and mesodermal cell types. Cross-reactivity was found with smooth muscledesmin.
Form
Liquid
Concentration
1 mg/mL
Isotype
IgM
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blotting analysis of VIM in whole cell lysate of LEP-19 (human fibroblast cell line) (1, 3) and NIH/3T3 (mouse embryonal fibroblast cell line) (2, 4).
Lane 1, 2 : Immunostaining with VIM monoclonal antibody, clone VI-01 (Cat # MAB3880).
Lane 3, 4 : Immunostaining with VIM monoclonal antibody, clone VI-RE/1.Immunocytochemistry
Immunofluorescence
Immunofluorescence staining of RBL (rat basophilic cell line) with VIM monoclonal antibody, clone VI-01 (Cat # MAB3880) conjugated with Dyomics 547. Nuclei are stained with DAPI (blue).Immunofluorescence
Immunofluorescence staining of NIH/3T3 (mouse embryonal fibroblast cell line) with VIM monoclonal antibody, clone VI-01 (Cat # MAB3880). Nuclei are stained with DAPI (blue).Immunoprecipitation
Immunoprecipitation of VIM from HeLa cell lysate and detection by VIM monoclonal antibody, clone VI-01 (Cat # MAB3880).
IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated.
MW of VIM is 57 kDa.
Lr = lysate (reducing conditions). Lnr = lysate (non-reducing conditions). IPr = immunoprecipitate (reducing conditions). IPnr = immunoprecipitate (non-reducing conditions). -
Gene Info — VIM
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Publication Reference
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Molecular mechanisms of improved adhesion and growth of an endothelial cell line cultured on polystyrene implanted with fluorine ions.
Bacáková L, Mares V, Lisá V, Svorcík V.
Biomaterials 2000 Jun; 21(11):1173.
Application：ELISA, Cow, CPAE cells.
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Fluorine ion-implanted polystyrene improves growth and viability of vascular smooth muscle cells in culture.
Bacáková L, Mares V, Bottone MG, Pellicciari C, Lisá V, Svorcík V.
Journal of Biomedical Materials Research 2000 Mar; 49(3):369.
Application：IF, Rat, Aortic smooth muscle cells.
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A common antigenic determinant of vimentin and desmin defined by monoclonal antibody.
Draberova E, Draber P, Havlicek F, Viklicky V.
Folia Biologica 1986 Jan; 32(5):295.
Application：IF, IHC-Fr, WB-Ce, RIA, Human, Rabbit, BHK-21, LEP cells, Human brain, Human skin, Human stomach Rabbit uterus.
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Molecular mechanisms of improved adhesion and growth of an endothelial cell line cultured on polystyrene implanted with fluorine ions.
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