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Last updated: 2016/12/4
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VIM monoclonal antibody, clone VI-01

  • Catalog # : MAB3880
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against native VIM.
  • Immunogen:
  • Native purified VIM from porcine brain cold stable proteins after depolymerization of microtubules.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 57
  • Reactivity:
  • Mammals, Pig
  • Specificity:
  • This antibody reacts with vimentin, a 57 KDa intermediate filament expressed in varietyof mesenchymal and mesodermal cell types. Cross-reactivity was found with smooth muscledesmin.
  • Form:
  • Liquid
  • Concentration:
  • 1 mg/mL
  • Isotype:
  • IgM
  • Recommend Usage:
  • The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. Do not freeze.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blotting analysis of VIM in whole cell lysate of LEP-19 (human fibroblast cell line) (1, 3) and NIH/3T3 (mouse embryonal fibroblast cell line) (2, 4).
    Lane 1, 2 : Immunostaining with VIM monoclonal antibody, clone VI-01 (Cat # MAB3880).
    Lane 3, 4 : Immunostaining with VIM monoclonal antibody, clone VI-RE/1.
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence staining of RBL (rat basophilic cell line) with VIM monoclonal antibody, clone VI-01 (Cat # MAB3880) conjugated with Dyomics 547. Nuclei are stained with DAPI (blue).
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence staining of NIH/3T3 (mouse embryonal fibroblast cell line) with VIM monoclonal antibody, clone VI-01 (Cat # MAB3880). Nuclei are stained with DAPI (blue).
  • Immunoprecipitation
  • Immunoprecipitation
  • Immunoprecipitation of VIM from HeLa cell lysate and detection by VIM monoclonal antibody, clone VI-01 (Cat # MAB3880).
    IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated.
    MW of VIM is 57 kDa.
    Lr = lysate (reducing conditions). Lnr = lysate (non-reducing conditions). IPr = immunoprecipitate (reducing conditions). IPnr = immunoprecipitate (non-reducing conditions).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunocytochemistry
  • Gene Information
  • Gene Name:
  • VIM
  • Gene Alias:
  • -
  • Gene Description:
  • vimentin
  • Other Designations:
  • -
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