HLA-G monoclonal antibody, clone MEM-G/9
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More Files
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Immunofluorescence
Immunofluorescence staining of HLA-G1 transfectants (LCL-HLA-G1) using HLA-G monoclonal antibody, clone MEM-G/9 (Cat # MAB3855) Alexa Fluor® 488 Fab-fragment.
Immunoprecipitation
Immunoprecipitation of HLA-G from HLA-G1 transfectants (LCL-HLA-G1) by HLA-G monoclonal antibody, clone MEM-G/9 (Cat # MAB3855) and protein G. HLA-G was detected by HLA-G monoclonal antibody, clone 4H84 (Cat # MAB3858) and goat anti-mouse HRP in cell lysate (Lane 1) and in the immunoprecipitate (Lane 2).
Flow Cytometry
Double surface staining of HLA-G1 transfectants (viable cells gate) using HLA-G monoclonal antibody, clone MEM-G/9 (Cat # MAB3855) - APC and HLA-G monoclonal antibody, clone MEM-G/11 (Cat # MAB3856) - FITC.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant HLA-G.
Immunogen
Recombinant protein corresponding to human HLA-G.
Host
Mouse
Reactivity
Human
Specificity
This antibody reacts with native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (10 ug/mL)
Flow Cytometry (1-5 ug/mL)
Immunocytochemistry
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoprecipitation
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunofluorescence
Immunofluorescence staining of HLA-G1 transfectants (LCL-HLA-G1) using HLA-G monoclonal antibody, clone MEM-G/9 (Cat # MAB3855) Alexa Fluor® 488 Fab-fragment.Immunoprecipitation
Immunoprecipitation of HLA-G from HLA-G1 transfectants (LCL-HLA-G1) by HLA-G monoclonal antibody, clone MEM-G/9 (Cat # MAB3855) and protein G. HLA-G was detected by HLA-G monoclonal antibody, clone 4H84 (Cat # MAB3858) and goat anti-mouse HRP in cell lysate (Lane 1) and in the immunoprecipitate (Lane 2).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Double surface staining of HLA-G1 transfectants (viable cells gate) using HLA-G monoclonal antibody, clone MEM-G/9 (Cat # MAB3855) - APC and HLA-G monoclonal antibody, clone MEM-G/11 (Cat # MAB3856) - FITC. -
Gene Info — HLA-G
Entrez GeneID
3135Gene Name
HLA-G
Gene Alias
MHC-G
Gene Description
major histocompatibility complex, class I, G
Gene Ontology
HyperlinkGene Summary
HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq
Other Designations
HLA class I molecule|HLA-G histocompatibility antigen, class I, G|MHC class I antigen|OTTHUMP00000039485|b2 microglobulin
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Interactome
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Pathway
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Disease
- Abortion
- Anemia
- Arthritis
- Asthma
- Autoimmune Diseases
- Behcet Syndrome
- Birth Weight
- Bronchial Hyperreactivity
- Carcinoma
+ View More Disease
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Publication Reference
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Regulatory role of tryptophan degradation pathway in HLA-G expression by human monocyte-derived dendritic cells.
Lopez AS, Alegre E, LeMaoult J, Carosella E, Gonzalez A.
Molecular Immunology 2006 Feb; 43(14):2151.
Application：Cap Ab, ELISA, Human, Human monocyte-derived dendritic cells.
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The CD85J/leukocyte inhibitory receptor-1 distinguishes between conformed and beta 2-microglobulin-free HLA-G molecules.
Gonen-Gross T, Achdout H, Arnon TI, Gazit R, Stern N, Horejsi V, Goldman-Wohl D, Yagel S, Mandelboim O.
Journal of Immunology 2005 Oct; 175(8):4866.
Application：IP, Human, Jeg3 cells.
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Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules.
Menier C, Saez B, Horejsi V, Martinozzi S, Krawice-Radanne I, Bruel S, Le Danff C, Reboul M, Hilgert I, Rabreau M, Larrad ML, Pla M, Carosella ED, Rouas-Freiss N.
Human Immunology 2003 Mar; 64(3):315.
Application：Flow Cyt, IHC-Fr, Human, LCL, JEG-3, K-562 cells, trophoblast tissue.
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Regulatory role of tryptophan degradation pathway in HLA-G expression by human monocyte-derived dendritic cells.
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