Mouse monoclonal antibody raised against recombinant HLA-G.
Recombinant protein corresponding to human HLA-G.
This antibody reacts with native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.
ELISA (10 ug/mL) Flow Cytometry (1-5 ug/mL) Immunocytochemistry Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) Immunoprecipitation The optimal working dilution should be determined by the end user.
In PBS, pH 7.4 (0.09% sodium azide)
Store at 4°C. Do not freeze. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunofluorescence staining of HLA-G1 transfectants (LCL-HLA-G1) using HLA-G monoclonal antibody, clone MEM-G/9 (Cat # MAB3855) Alexa Fluor® 488 Fab-fragment.
Immunoprecipitation of HLA-G from HLA-G1 transfectants (LCL-HLA-G1) by HLA-G monoclonal antibody, clone MEM-G/9 (Cat # MAB3855) and protein G. HLA-G was detected by HLA-G monoclonal antibody, clone 4H84 (Cat # MAB3858) and goat anti-mouse HRP in cell lysate (Lane 1) and in the immunoprecipitate (Lane 2).
HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq
HLA class I molecule,HLA-G histocompatibility antigen, class I, G,MHC class I antigen,OTTHUMP00000039485,b2 microglobulin