CEACAM5 monoclonal antibody, clone CB30
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Specification
Product Description
Mouse monoclonal antibody raised against CEACAM5.
Immunogen
Human CEACAM5.
Host
Mouse
Theoretical MW (kDa)
180-200
Reactivity
Human
Specificity
This antibody recognizes CD66e (CEA; 180-200 KDa), a cell surface bound carcinoembryonic antigen mainly expressed on epithelial cells.
Form
Liquid
Isotype
IgG1
Recommend Usage
Flow Cytometry
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (10 ug/mL)
Immunoprecipitation
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry staining of human colorectal carcinoma (paraffin-embedded sections) with CEACAM5 monoclonal antibody, clone CB30 (Cat # MAB3848). Primary antibody dilution : 10 ug/mL .Immunoprecipitation
Flow Cytometry
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Gene Info — CEACAM5
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Interactome
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Publication Reference
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In vitro and in vivo footprint analysis of the promoter of carcinoembryonic antigen in colon carcinoma cells: effects of interferon gamma treatment.
Chen CJ, Li LJ, Maruya A, Shively JE.
Cancer Research 1995 Sep; 55(17):3873.
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Carcinoembryonic antigen gene family: molecular biology and clinical perspectives.
Thompson JA, Grunert F, Zimmermann W.
Journal of Clinical Laboratory Analysis 1991 Jan; 5(5):344.
Application:ELISA, Human, Human serum.
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Primary structure of human carcinoembryonic antigen (CEA) deduced from cDNA sequence.
Oikawa S, Nakazato H, Kosaki G.
Biochemical and Biophysical Research Communications 1987 Jan; 42(2):511.
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In vitro and in vivo footprint analysis of the promoter of carcinoembryonic antigen in colon carcinoma cells: effects of interferon gamma treatment.
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