CD8 monoclonal antibody, clone MEM-31
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More Files
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Flow Cytometry
Surface staining of human peripheral blood cells with CD8 monoclonal antibody, clone MEM-31 (Cat # MAB3844) conjugated with FITC.
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Specification
Product Description
Mouse monoclonal antibody raised against native CD8.
Immunogen
Native purified CD8 from human crude thymus membrane fraction.
Host
Mouse
Theoretical MW (kDa)
32-34
Reactivity
Human
Specificity
This antibody recognizes a conformationally-dependent epitope of CD8, a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. CD8 is a disulfide-linked dimer and exists as a CD8a homodimer or CD8a/b heterodimer (each monomer approx. 32-34 KDa).This antibody does not react with formaldehyde-fixed cells; negative in Western Blot application.
Form
Liquid
Isotype
IgG2a
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunoprecipitation
Flow Cytometry
Surface staining of human peripheral blood cells with CD8 monoclonal antibody, clone MEM-31 (Cat # MAB3844) conjugated with FITC. -
Gene Info — CD8A
Entrez GeneID
925Gene Name
CD8A
Gene Alias
CD8, Leu2, MAL, p32
Gene Description
CD8a molecule
Gene Ontology
HyperlinkGene Summary
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a corepressor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains, or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain isoforms. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
CD8 antigen alpha polypeptide|CD8 antigen, alpha polypeptide (p32)|Leu2 T-lymphocyte antigen|OKT8 T-cell antigen|T cell co-receptor|T-cell antigen Leu2|T-cell surface glycoprotein CD8 alpha chain|T-lymphocyte differentiation antigen T8/Leu-2|T8 T-cell ant
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Gene Info — CD8B
Entrez GeneID
926Gene Name
CD8B
Gene Alias
CD8B1, LYT3, Leu2, Ly3, MGC119115
Gene Description
CD8b molecule
Omim ID
186730Gene Ontology
HyperlinkGene Summary
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified. [provided by RefSeq
Other Designations
CD8 antigen, beta polypeptide (p37)|CD8 antigen, beta polypeptide 1 (p37)|CD8b antigen|OTTHUMP00000160761|T lymphocyte surface glycoprotein beta chain|T-cell surface glycoprotein CD8 beta chain
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Interactome
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Pathway
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Disease
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Publication Reference
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Coreceptor CD8-driven modulation of T cell antigen receptor specificity.
van den Berg HA, Wooldridge L, Laugel B, Sewell AK.
Journal of Theoretical Biology 2007 Nov; 249(2):395.
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CD8 Raft localization is induced by its assembly into CD8alpha beta heterodimers, Not CD8alpha alpha homodimers.
Pang DJ, Hayday AC, Bijlmakers MJ.
The Journal of Biological Chemistry 2007 May; 282(18):13884.
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Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74).
Horejsí V, Angelisová P, Bazil V, Kristofová H, Stoyanov S, Stefanová I, Hausner P, Vosecký M, Hilgert I.
Folia Biol (Praha) 1988 Jan; 34(1):23.
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Coreceptor CD8-driven modulation of T cell antigen receptor specificity.
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