Mouse monoclonal antibody raised against native CD5.
Immunogen:
Native purified CD5 from stimulated human leukocytes.
Host:
Mouse
Theoretical MW (kDa):
67
Reactivity:
Human
Specificity:
This antibody reacts with an extracellular epitope of CD5, a 67 kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B lymphocytes subset (B-1a lymphocytes).
Form:
Liquid
Purification:
Protein A affinity chromatography
Isotype:
IgG2a
Recommend Usage:
ELISA Flow Cytometry Immunohistochemistry (Frozen sections) Immunoprecipitation Western Blot The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS (15 mM sodium azide).
Storage Instruction:
Store at 4°C. Do not freeze. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Flow Cytometry analysis of human peripheral blood lymphocytes (PBL) stained with CD5 monoclonal antibody, clone CRIS1 (Cat # MAB3840) (dilution of purified antibody 1 ug/mL). Fig. A : Histogram - gated on lymphocytes, overlay with isotypic control (mouse IgG2a). Fig. B : Dot plot.