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Last updated: 2016/12/11
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Bcl2l11 monoclonal antibody, clone 5E5

  • Catalog # : MAB3705
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  • Specification
  • Product Description:
  • Rat monoclonal antibody raised against recombinant Bcl2l11.
  • Immunogen:
  • Recombinant GST fusion protein corresponding to mouse Bcl2l11.
  • Host:
  • Rat
  • Reactivity:
  • Human, Monkey, Mouse
  • Specificity:
  • Recognizes BimL.
  • Form:
  • Liquid
  • Isotype:
  • IgG2b
  • Recommend Usage:
  • The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.02% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • IP/Western blot analysis of Bim expression in (C) depicts the ability of Bcl2l11 monoclonal antibody, clone 5E5 (Cat # MAB3705) to immunoprecipitate BimL and BimEL from lysates of spleen and thymus from normal mice, but not from Bim-deficient mice.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunocytochemistry
  • Immunoprecipitation
  • Immunoprecipitation
  • IP/Western blot analysis of Bim expression in mouse (A) and human (B) cell lines. Bim proteins were immunoprecipitated from lysates of 107 cells with Bcl2l11 monoclonal antibody, clone 14A8 (Cat # MAB3704) and Bcl2l11 monoclonal antibody, clone 5E5 (Cat # MAB3705). Western blot analysis was performed with MAB3704 and MAB3705 followed by the addition of biotinylated mouse anti-rat IgG2b antibody and HRP-conjugated mouse anti-rat IgG2a antibody as the respective secondary antibodies, and ECL detection.
  • Flow Cytometry
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunocytochemistry
  • Flow Cytometry
  • Gene Information
  • Gene Name:
  • Bcl2l11
  • Gene Alias:
  • 1500006F24Rik,Bim,Bod
  • Gene Description:
  • BCL2-like 11 (apoptosis facilitator)
  • Other Designations:
  • BCL2-like 11 apoptosis facilitator,Bcl2 interacting mediator of cell death,OTTMUSP00000016362,OTTMUSP00000016364,OTTMUSP00000016365
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