Tubulin-beta monoclonal antibody, clone TU-06
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blotting analysis (reducing conditions) of HPB-ALL (human peripheral blood leukemia cell line).
Lane 1 : Negative control.
Lane 2, 3, 4, 5, 6 : Immunostaining with TUBB1 monoclonal antibody, clone TU-06 (Cat # MAB3656 ; dilution 0,5 ug/mL , 1 ug/mL , 2 ug/mL , 4 ug/mL , 5 ug/mL).
Immunofluorescence
Immunofluorescence staining of NIH/3T3 (mouse embryonal fibroblast cell line) using TUBB1 monoclonal antibody, clone TU-06 (Cat # MAB3656) (detection by Goat anti-mouse IgM Cy®5). Nucleus is stained with DAPI (blue).
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Specification
Product Description
Mouse monoclonal antibody raised against native Tubulin-beta.
Immunogen
Native purified porcine Tubulin-beta.
Host
Mouse
Reactivity
Pig
Specificity
This antibody recognizes an epitope (aa 81-95) on phylogenetically conserved N-terminal structural domain of beta-tubulin (recognizes all beta-tubulin isoforms) in various species.
Form
Liquid
Concentration
1 mg/mL
Isotype
IgM
Recommend Usage
Immunocytochemistry (2 ug/mL)
Western Blot (1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 8.0 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blotting analysis (reducing conditions) of HPB-ALL (human peripheral blood leukemia cell line).
Lane 1 : Negative control.
Lane 2, 3, 4, 5, 6 : Immunostaining with TUBB1 monoclonal antibody, clone TU-06 (Cat # MAB3656 ; dilution 0,5 ug/mL , 1 ug/mL , 2 ug/mL , 4 ug/mL , 5 ug/mL).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunofluorescence
Immunofluorescence staining of NIH/3T3 (mouse embryonal fibroblast cell line) using TUBB1 monoclonal antibody, clone TU-06 (Cat # MAB3656) (detection by Goat anti-mouse IgM Cy®5). Nucleus is stained with DAPI (blue). -
Publication Reference
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Engineered early embryonic cardiac tissue retains proliferative and contractile properties of developing embryonic myocardium.
Tobita K, Liu LJ, Janczewski AM, Tinney JP, Nonemaker JM, Augustine S, Stolz DB, Shroff SG, Keller BB.
American Journal of Physiology. Heart and Circulatory Physiology 2006 Oct; 291(4):H1829.
Application：IF, Chicken, Embryonic cardiac tissue.
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Distinct localization of a beta-tubulin epitope in the Tetrahymena thermophila and Paramecium caudatum cortex.
Libusová L, Sulimenko T, Sulimenko V, Janisch R, Hozák P, Dráber P.
Protoplasma 2005 Oct; 225(3-4):157.
Application：IEM, IF, WB, Mouse, Parasite, NIH/3T3 cells, T. thermophila cortical region.
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Par6alpha signaling controls glial-guided neuronal migration.
Solecki DJ, Model L, Gaetz J, Kapoor TM, Hatten ME.
Nature Neuroscience 2004 Nov; 7(11):1195.
Application：ICC, Mouse, Cerebellar granule neurons.
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Engineered early embryonic cardiac tissue retains proliferative and contractile properties of developing embryonic myocardium.
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