GST monoclonal antibody, clone S-tag-05
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Specification
Product Description
Mouse monoclonal antibody raised against GST.
Immunogen
Glutathione-S-Transferase (GST) fusion protein.
Host
Mouse
Specificity
This antibody is a mouse monoclonal antibody with high affinity to theglutathione-S-transferase (GST) from Schistosoma japonicum. This purified antibody is suitable for detecting fusion proteins containing the GST-Tag sequence expressed in E. coli, yeast, mammalian, and in vitro transcription/translation systems. Recognizes fusion proteins in all species.
Form
Liquid
Isotype
IgG2b
Recommend Usage
Western Blot (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Isolation of Glutathione-S-Transferase (GST) overexpressed in E. coli.
Western blot was immunostained by GST monoclonal antibody, clone S-tag-05 (Cat # MAB3651).
Lane 1 : Affinity purification of GST from bacterial cell lysate using commercial sorbent Glutathione-Sepharose.
Lane 2 : Affinity purification of GST from bacterial cell lysate using immunosorbent prepared from anti-GST (S-tag-02).Immunoprecipitation
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Publication Reference
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Persistent transactivation of EGFR and ErbB2/HER2 by protease-activated receptor-1 promotes breast carcinoma cell invasion.
Arora P, Cuevas BD, Russo A, Johnson GL, Trejo J.
Oncogene 2008 Jul; 27(32):4434.
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Signaling through ShcA is required for transforming growth factor beta- and Neu/ErbB-2-induced breast cancer cell motility and invasion.
Northey JJ, Chmielecki J, Ngan E, Russo C, Annis MG, Muller WJ, Siegel PM.
Molecular and Cellular Biology 2008 May; 28(10):3162.
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Overexpression of ERBB-2 was more frequently detected in malignant than benign pheochromocytomas by multiplex ligation-dependent probe amplification and immunohistochemistry.
Yuan W, Wang W, Cui B, Su T, Ge Y, Jiang L, Zhou W, Ning G.
Endocrine-Related Cancer 2008 Mar; 15(1):343.
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Persistent transactivation of EGFR and ErbB2/HER2 by protease-activated receptor-1 promotes breast carcinoma cell invasion.
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