This antibody is a mouse monoclonal antibody with high affinity to theglutathione-S-transferase (GST) from Schistosoma japonicum. This purified antibody is suitable for detecting fusion proteins containing the GST-Tag sequence expressed in E. coli, yeast, mammalian, and in vitro transcription/translation systems. Recognizes fusion proteins in all species.
Western Blot (1 ug/mL) The optimal working dilution should be determined by the end user.
In PBS, pH 7.4 (0.09% sodium azide)
Store at 4°C. Do not freeze.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Isolation of Glutathione-S-Transferase (GST) overexpressed in E. coli. Western blot was immunostained by GST monoclonal antibody, clone S-tag-05 (Cat # MAB3651). Lane 1 : Affinity purification of GST from bacterial cell lysate using commercial sorbent Glutathione-Sepharose. Lane 2 : Affinity purification of GST from bacterial cell lysate using immunosorbent prepared from anti-GST (S-tag-02).