STAT1 (phospho S727) monoclonal antibody, clone PSM1
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More Files
- More Functions
Immunoprecipitation
Western Blotting analysis (reducing conditions) of phosphorylated STAT1 (Ser727) in IFN-gamma treated HeLa (human cervix carcinoma cell line) using STAT1 (phospho S727) monoclonal antibody, clone PSM1 (Cat # MAB3641).
Lane 1 : Immunoprecipitated material by STAT1 monoclonal antibody, clone SM2.
Lane 2 : original whole cell lysate.
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic phosphopeptide of STAT1.
Immunogen
Synthetic phosphopeptide corresponding to amino acids 721-733 residues surrounding S727 of STAT1.
Host
Mouse
Theoretical MW (kDa)
91
Reactivity
Human
Specificity
This antibody recognizes transcriptional factor STAT1 (91 KDa) activated byphosphorylation at Ser727.
Form
Liquid
Isotype
IgG1
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunoprecipitation
Western Blotting analysis (reducing conditions) of phosphorylated STAT1 (Ser727) in IFN-gamma treated HeLa (human cervix carcinoma cell line) using STAT1 (phospho S727) monoclonal antibody, clone PSM1 (Cat # MAB3641).
Lane 1 : Immunoprecipitated material by STAT1 monoclonal antibody, clone SM2.
Lane 2 : original whole cell lysate. -
Publication Reference
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ERK and the F-box protein betaTRCP target STAT1 for degradation.
Soond SM, Townsend PA, Barry SP, Knight RA, Latchman DS, Stephanou A.
The Journal of Biological Chemistry 2008 Jun; 283(23):16077.
Application：WB, Mouse, MEF cells.
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Malignant melanoma associates with deficient IFN-induced STAT 1 phosphorylation.
Kovarik J, Boudny V, Kocak I, Lauerova L, Fait V, Vagundova M.
International Journal of Molecular Medicine 2003 Sep; 12(3):335.
Application：WB-Ce, Human, Malignant melanoma cells.
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Interferon inducibility of STAT 1 activation and its prognostic significance in melanoma patients.
Boudný V, Kocák I, Lauerová L, Kovarík J.
Folia Biol (Praha) 2003 Jan; 49(4):142.
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ERK and the F-box protein betaTRCP target STAT1 for degradation.
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