Neurofilament heavy protein monoclonal antibody, clone NF-01
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry staining of human cerebellum (paraffin-embedded sections) with Neurofilament heavy protein monoclonal antibody, clone NF-01 (Cat # MAB3638).
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Specification
Product Description
Mouse monoclonal antibody raised against native Neurofilament heavy protein.
Immunogen
Native purified porcine Neurofilament heavy protein.
Host
Mouse
Theoretical MW (kDa)
210
Reactivity
Mammals, Pig
Specificity
This antibody recognizes a phosphorylated epitope on heavy neurofilament protein (210 KDa) of various species. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.
Form
Liquid
Isotype
IgG1
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry staining of human cerebellum (paraffin-embedded sections) with Neurofilament heavy protein monoclonal antibody, clone NF-01 (Cat # MAB3638).Immunocytochemistry
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Publication Reference
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A systematic review and meta-analysis of CSF neurofilament protein levels as biomarkers in dementia.
Petzold A, Keir G, Warren J, Fox N, Rossor MN.
Neuro-Degenerative Diseases 2007 Jun; 4(2-3):185.
Application：ELISA, Human, Cerebrospinal fluid from patients with neurodegenerative dementias.
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Plasma neurofilament heavy chain levels in Huntington's disease.
Wild EJ, Petzold A, Keir G, Tabrizi SJ.
Neuroscience Letters 2007 May; 417(3):231.
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Expression of phosphorylated high molecular weight neurofilament protein (NF-H) and vimentin in human developing dorsal root ganglia and spinal cord.
Lukas Z, Draber P, Bucek J, Draberova E, Viklicky V, Dolezel S.
Histochemistry 1993 Dec; 100(6):495.
Application：IF, IHC-Fr, WB-Ce, Human, Dorsal root ganglia, Spinal cords, U-1285 cells.
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A systematic review and meta-analysis of CSF neurofilament protein levels as biomarkers in dementia.
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