5-bromodeoxyuridine (BrdU) monoclonal antibody, clone MoBu-1
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry staining of bromodeoxyuridine-labeled cells (chick embryo ; paraffin-embedded sections) with 5-bromodeoxyuridine (BrdU) monoclonal antibody, clone MoBu-1 (Cat # MAB3635).
Flow Cytometry
Flow cytometric analysis of 5-bromodeoxyuridin (BrdU) incorporation in CEM (human acute lymphoblastic leukemia cell line) using 5-bromodeoxyuridine (BrdU) monoclonal antibody, clone MoBu-1 (Cat # MAB3635) (detection by Goat anti-mouse IgG1 FITC).
The individual cell cycle phases (S-, G1-, G2/M-phase) are indicated in the figure.
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Specification
Product Description
Mouse monoclonal antibody raised against 5-bromodeoxyuridine (BrdU).
Immunogen
5-bromodeoxyuridine (BrdU) conjugated with Hemocyanin.
Host
Mouse
Specificity
This antibody reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. This antibody is also useful for detecting proliferating cells by flowcytometry or immunofluorescence staining. It reacts also specifically with 5-bromouridine (BrU). Does not react with Edu.
Form
Liquid
Concentration
1 mg/mL
Isotype
IgG1
Recommend Usage
Immunocytochemistry (2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry staining of bromodeoxyuridine-labeled cells (chick embryo ; paraffin-embedded sections) with 5-bromodeoxyuridine (BrdU) monoclonal antibody, clone MoBu-1 (Cat # MAB3635).Immunocytochemistry
Flow Cytometry
Flow cytometric analysis of 5-bromodeoxyuridin (BrdU) incorporation in CEM (human acute lymphoblastic leukemia cell line) using 5-bromodeoxyuridine (BrdU) monoclonal antibody, clone MoBu-1 (Cat # MAB3635) (detection by Goat anti-mouse IgG1 FITC).
The individual cell cycle phases (S-, G1-, G2/M-phase) are indicated in the figure. -
Publication Reference
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Pre-ribosomal RNA is processed in permeabilised cells at the site of transcription.
Stanek D, Kiss T, Raska I.
European Journal of Cell Biology 2000 Mar; 79(3):202.
Application：IF, Human, HeLa cells.
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Induction of hyperplasia and increased DNA content in the uterus of immature rats exposed to coumestrol.
Ashby J, Tinwell H, Soames A, Foster J.
Environmental Health Perspectives 1999 Oct; 107(10):819.
Application：IHC-P, Rat, Longitudinal sections of uterus.
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Hyperthermia in the chick embryo: HSP and possible mechanisms of developmental defects.
Buckiova D, Kubinova L, Soukup A, Jelinek R, Brown NA.
The International Journal of Developmental Biology 1998 Jul; 42(5):737.
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Pre-ribosomal RNA is processed in permeabilised cells at the site of transcription.
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