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Last updated: 2022/5/15
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5-bromodeoxyuridine (BrdU) monoclonal antibody, clone MoBu-1 

  • Catalog # : MAB3635
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against 5-bromodeoxyuridine (BrdU).
  • Immunogen:
  • 5-bromodeoxyuridine (BrdU) conjugated with Hemocyanin.
  • Host:
  • Mouse
  • Specificity:
  • This antibody reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. This antibody is also useful for detecting proliferating cells by flowcytometry or immunofluorescence staining. It reacts also specifically with 5-bromouridine (BrU). Does not react with Edu.
  • Form:
  • Liquid
  • Concentration:
  • 1 mg/mL
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Immunocytochemistry (2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. Do not freeze.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry staining of bromodeoxyuridine-labeled cells (chick embryo ; paraffin-embedded sections) with 5-bromodeoxyuridine (BrdU) monoclonal antibody, clone MoBu-1 (Cat # MAB3635).
  • Immunocytochemistry
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometry analysis of 5-bromodeoxyuridin (BrdU) incorporation in CEM (human acute lymphoblastic leukemia cell line) using 5-bromodeoxyuridine (BrdU) monoclonal antibody, clone MoBu-1 (Cat # MAB3635) (detection by Goat anti-mouse IgG1 FITC).
    The individual cell cycle phases (S-, G1-, G2/M-phase) are indicated in the figure.
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunocytochemistry
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