HLA-E monoclonal antibody, clone MEM-E/07
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant HLA-E.
Immunogen
Recombinant protein corresponding to human HLA-E.
Host
Mouse
Reactivity
Human
Specificity
This antibody recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells. The published results revealed that This antibody cross-reacts with some classical MHC Class I molecules (MHC Class Ia) : HLA-B7 (strongly), HLA-B8 (moderately), HLA-B27, -B44 (weakly).
Form
Liquid
Purification
Protein A affinity chromatography
Purity
>95% (by SDS-PAGE)
Isotype
IgG1
Recommend Usage
Flow Cytometry (10 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (10 ug/mL)
Immunoprecipitation (10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoprecipitation
Flow Cytometry
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Gene Info — HLA-E
Entrez GeneID
3133Gene Name
HLA-E
Gene Alias
DKFZp686P19218, EA1.2, EA2.1, HLA-6.2, MHC, QA1
Gene Description
major histocompatibility complex, class I, E
Omim ID
143010Gene Ontology
HyperlinkGene Summary
HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. [provided by RefSeq
Other Designations
HLA class I histocompatibility antigen, E alpha chain|MHC HLA-E alpha-1|MHC HLA-E alpha-2.1|MHC class I antigen|OTTHUMP00000029214|lymphocyte antigen
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Interactome
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Pathway
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Disease
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Publication Reference
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HLA-E surface expression is independent of the availability of HLA class I signal sequence-derived peptides in human tumor cell lines.
Palmisano GL, Contardi E, Morabito A, Gargaglione V, Ferrara GB, Pistillo MP.
Human Immunology 2005 Jan; 66(1):1.
Application:Flow Cyt, Human, MG63, CACO2, MCF-7, CHP212, U2OS, HELA, AEH, 221-A3, 221-Cw1, 221-G, 220-TPN, 221-WT cells.
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HLA-E surface expression is independent of the availability of HLA class I signal sequence-derived peptides in human tumor cell lines.
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