Mouse monoclonal antibody raised against recombinant HLA-E.
Recombinant protein corresponding to human HLA-E.
This antibody recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells. The published results showed that This antibody cross-reacts with some classical MHC ClassI molecules (HLA-A3, -A11, -B7). However, the recent Workshop I Session on the 3rd International Conference on HLA-G (Paris, July 2003) confirmed that This antibody exhibits much broader cross-reactivity classical MHC Class I antigens, namely with HLA-A24, -A32,-B8, -B15, -B27, -B35, -B44, -B54, -C3, -C4, -C5, -C7.
The optimal working dilution should be determined by the end user.
In TBS, pH 8.0 (0.09% sodium azide)
Store at 4°C. Do not freeze.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. [provided by RefSeq
HLA class I histocompatibility antigen, E alpha chain,MHC HLA-E alpha-1,MHC HLA-E alpha-2.1,MHC class I antigen,OTTHUMP00000029214,lymphocyte antigen