Mouse monoclonal antibody raised against native CD59.
Native Thymocytes and T lymphocytes.
Theoretical MW (kDa):
This antibody reacts with well defined epitope (around L33) on CD59 (Protectin), a 18-20 KDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on all hematopoietic cells; it is widely present on cells in all tissues. This antibody does not compete with most other CD59 antibodies.
Protein A purification
Flow Cytometry (1 ug/mL) Western Blot (1-2 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5 ug/mL) The optimal working dilution should be determined by the end user.
In PBS, pH 7.4 (0.09% sodium azide)
Store at 4°C. Do not freeze. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blotting analysis (non-reducing conditions) of whole cell lysate of HPB-ALL (human peripheral blood T cell leukemia cell line) using CD59 monoclonal antibody, clone MEM-43/5 (Cat # MAB3628). Lane 1 : Original cell lysate. Lane 2 : Material immunoprecipitated with CD59 monoclonal antibody, clone MEM-43/5 (Cat # MAB3628).
This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq
20 kDa homologous restriction factor,CD59 antigen,CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344),CD59 glycoprotein,Ly-6-like protein,T cell-activating protein,human leukocyte antigen MIC11,lymphocytic a