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Last updated: 2017/10/15
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CD59 monoclonal antibody, clone MEM-43/5

  • Catalog # : MAB3628
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against native CD59.
  • Immunogen:
  • Native Thymocytes and T lymphocytes.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 18-20
  • Reactivity:
  • Human, Mouse
  • Specificity:
  • This antibody reacts with well defined epitope (around L33) on CD59 (Protectin), a 18-20 KDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on all hematopoietic cells; it is widely present on cells in all tissues. This antibody does not compete with most other CD59 antibodies.
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG2b
  • Recommend Usage:
  • Flow Cytometry (1 ug/mL)
    Western Blot (1-2 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. Do not freeze.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot
  • Western Blot
  • Western Blotting analysis (non-reducing conditions) of whole cell lysate of HPB-ALL (human peripheral blood T cell leukemia cell line) using CD59 monoclonal antibody, clone MEM-43/5 (Cat # MAB3628).
    Lane 1 : Original cell lysate.
    Lane 2 : Material immunoprecipitated with CD59 monoclonal antibody, clone MEM-43/5 (Cat # MAB3628).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunoprecipitation
  • Flow Cytometry
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunoprecipitation
  • Flow Cytometry
  • Gene Information
  • Entrez GeneID:
  • 966
  • Gene Name:
  • CD59
  • Gene Alias:
  • 16.3A5,1F5,EJ16,EJ30,EL32,FLJ38134,FLJ92039,G344,HRF-20,HRF20,MAC-IP,MACIF,MEM43,MGC2354,MIC11,MIN1,MIN2,MIN3,MIRL,MSK21,p18-20
  • Gene Description:
  • CD59 molecule, complement regulatory protein
  • Gene Summary:
  • This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq
  • Other Designations:
  • 20 kDa homologous restriction factor,CD59 antigen,CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344),CD59 glycoprotein,Ly-6-like protein,T cell-activating protein,human leukocyte antigen MIC11,lymphocytic a
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