CD59 monoclonal antibody, clone MEM-43/5
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Specification
Product Description
Mouse monoclonal antibody raised against native CD59.
Immunogen
Native Thymocytes and T lymphocytes.
Host
Mouse
Theoretical MW (kDa)
18-20
Reactivity
Human, Mouse
Specificity
This antibody reacts with well defined epitope (around L33) on CD59 (Protectin), a 18-20 KDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on all hematopoietic cells; it is widely present on cells in all tissues. This antibody does not compete with most other CD59 antibodies.
Form
Liquid
Purification
Protein A purification
Isotype
IgG2b
Recommend Usage
Flow Cytometry (1 ug/mL)
Western Blot (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blotting analysis (non-reducing conditions) of whole cell lysate of HPB-ALL (human peripheral blood T cell leukemia cell line) using CD59 monoclonal antibody, clone MEM-43/5 (Cat # MAB3628).
Lane 1 : Original cell lysate.
Lane 2 : Material immunoprecipitated with CD59 monoclonal antibody, clone MEM-43/5 (Cat # MAB3628).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoprecipitation
Flow Cytometry
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Gene Info — CD59
Entrez GeneID
966Gene Name
CD59
Gene Alias
16.3A5, 1F5, EJ16, EJ30, EL32, FLJ38134, FLJ92039, G344, HRF-20, HRF20, MAC-IP, MACIF, MEM43, MGC2354, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, p18-20
Gene Description
CD59 molecule, complement regulatory protein
Omim ID
107271Gene Ontology
HyperlinkGene Summary
This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq
Other Designations
20 kDa homologous restriction factor|CD59 antigen|CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344)|CD59 glycoprotein|Ly-6-like protein|T cell-activating protein|human leukocyte antigen MIC11|lymphocytic a
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Interactome
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Pathway
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Disease
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Publication Reference
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CD59a is the primary regulator of membrane attack complex assembly in the mouse.
Baalasubramanian S, Harris CL, Donev RM, Mizuno M, Omidvar N, Song WC, Morgan BP.
Journal of Immunology 2004 Sep; 173(6):3684.
Application:ELISA, Flow Cyt, IF, WB, Mouse, EL4 cells, Erythrocytes, Mouse serum, Tissues.
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Mutational analysis of the active site and antibody epitopes of the complement-inhibitory glycoprotein, CD59.
Bodian DL, Davis SJ, Morgan BP, Rushmere NK.
The Journal of Experimental Medicine 1997 Feb; 185(3):507.
Application:WB-Tr, Mouse, CHO cells.
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CD59 costimulation of T cell activation. CD58 dependence and requirement for glycosylation.
Menu E, Tsai BC, Bothwell AL, Sims PJ, Bierer BE.
Journal of Immunology 1994 Sep; 153(6):2444.
Application:Flow Cyt, IF, Mouse, CHO cells.
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CD59a is the primary regulator of membrane attack complex assembly in the mouse.
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