Kinesin (heavy chain) monoclonal antibody, clone KN-02
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Specification
Product Description
Mouse monoclonal antibody raised against native Kinesin (heavy chain).
Immunogen
Native purified porcine Kinesin (heavy chain).
Host
Mouse
Reactivity
Human, Mouse, Pig, Rat
Specificity
This antibody recognizes heavy chain of conventional kinesin associated withvesicles and with lower affinity with denaturated molecule. Epitope is located in coiled-coilstalk domain. It stains Western blots of kinesin-enriched preparations. Epitope mapping (by limited proteolysis of partially purified porcine kinesin) followed by immunoblotting has revealed that antibodies MAB3620, MAB3621 and MAB3622 react with differentsets of fragments. This antibody does not react with kinesin bound to taxol-stabilized microtubules.
Form
Liquid
Concentration
1 mg/mL
Isotype
IgM
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunocytochemistry
Immunofluorescence
Immunofluorescence staining of vesicles (red) in RBL-2H3 (rat basophilic leukemia cell line) using Kinesin (heavy chain) monoclonal antibody, clone KN-02 (Cat # MAB3621). Nuclei were stained with DAPI (blue). -
Publication Reference
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Preparation of human recombinant kinesin heavy chain and epitope mapping of its structural domains.
Malcová-Janatová I, Richterová V, Dráber P, Hasek J.
Folia Microbiologica 2004 Nov; 49(6):665.
Application:WB-Ce, E. coli, BL21DE3Star cells.
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Monoclonal antibodies KN-02 and KN-03 against the heavy chain of kinesin.
Macůrek L, Dráberová E, Richterová V, Böhm KJ, Dráber P.
Hybridoma and Hybridomics 2002 Dec; 21(6):457.
Application:IF, IP, WB-Re, Mouse, NIH/3T3 cells, Recombinant protein.
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Preparation of human recombinant kinesin heavy chain and epitope mapping of its structural domains.
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