Mouse monoclonal antibody raised against synthetic peptide of HRAS.
A synthetic peptide corresponding to human HRAS.
Theoretical MW (kDa):
This antibody reacts with human HRAS, an ubiquitously expressed 21 KDa intracellular protein. Although reactivity with other species has not been determined, it is probable as the epitope is highly conserved among animals.
Western Blot (0.5-1 ug/mL) The optimal working dilution should be determined by the end user.
In PBS, pH 7.4 (0.09% sodium azide)
Store at 4°C. Do not freeze. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blotting analysis (reducing conditions) of HRAS in whole cell lysate using HRAS monoclonal antibody, clone H-RAS-03 (Cat # MAB3617). Lane 1 : HeLa, human cervix carcinoma cell line. Lane 2 : K-567, human leukemia cell line. Lane 3 : RAJI, human Burkitt lymphoma cell line.
This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene. [provided by RefSeq
GTP- and GDP-binding peptide B,GTPase HRas,Ha-Ras1 proto-oncoprotein,OTTHUMP00000162769,OTTHUMP00000166053,OTTHUMP00000166055,Ras family small GTP binding protein H-Ras,c-has/bas p21 protein,c-ras-Ki-2 activated oncogene,p19 H-RasIDX protein,transformatio