HRAS monoclonal antibody, clone H-RAS-03
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of HRAS.
Immunogen
A synthetic peptide corresponding to human HRAS.
Sequence
DIHQYREQIKRVKDSDDC
Host
Mouse
Theoretical MW (kDa)
21
Reactivity
Human
Specificity
This antibody reacts with human HRAS, an ubiquitously expressed 21 KDa intracellular protein. Although reactivity with other species has not been determined, it is probable as the epitope is highly conserved among animals.
Form
Liquid
Concentration
1 mg/mL
Isotype
IgG1
Recommend Usage
Western Blot (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blotting analysis (reducing conditions) of HRAS in whole cell lysate using HRAS monoclonal antibody, clone H-RAS-03 (Cat # MAB3617).
Lane 1 : HeLa, human cervix carcinoma cell line.
Lane 2 : K-567, human leukemia cell line.
Lane 3 : RAJI, human Burkitt lymphoma cell line. -
Gene Info — HRAS
Entrez GeneID
3265Gene Name
HRAS
Gene Alias
C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, K-RAS, N-RAS, RASH1
Gene Description
v-Ha-ras Harvey rat sarcoma viral oncogene homolog
Gene Ontology
HyperlinkGene Summary
This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene. [provided by RefSeq
Other Designations
GTP- and GDP-binding peptide B|GTPase HRas|Ha-Ras1 proto-oncoprotein|OTTHUMP00000162769|OTTHUMP00000166053|OTTHUMP00000166055|Ras family small GTP binding protein H-Ras|c-has/bas p21 protein|c-ras-Ki-2 activated oncogene|p19 H-RasIDX protein|transformatio
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Interactome
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Pathway
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Disease
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Publication Reference
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The Hsp90 molecular chaperone: an open and shut case for treatment.
Pearl LH, Prodromou C, Workman P.
The Biochemical Journal 2008 Mar; 410(3):439.
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Expressed as the sole Hsp90 of yeast, the alpha and beta isoforms of human Hsp90 differ with regard to their capacities for activation of certain client proteins, whereas only Hsp90beta generates sensitivity to the Hsp90 inhibitor radicicol.
Millson SH, Truman AW, Rácz A, Hu B, Panaretou B, Nuttall J, Mollapour M, Söti C, Piper PW.
The FEBS Journal 2007 Sep; 274(17):4453.
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Blind cavefish and heat shock protein chaperones: a novel role for hsp90alpha in lens apoptosis.
Hooven TA, Yamamoto Y, Jeffery WR.
The International Journal of Developmental Biology 2004 Oct; 48(8-9):731.
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The Hsp90 molecular chaperone: an open and shut case for treatment.
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