Mouse monoclonal antibody raised against native GFAP.
Immunogen:
Native purified porcine GFAP.
Host:
Mouse
Theoretical MW (kDa):
55
Reactivity:
Human, Pig
Specificity:
This antibody exclusively reacts with intact GFAP molecules. GFAP is the principal marker of astroglial cells in the central nervous system; it is specifically expressed in satellite cells in peripheral ganglia and in non myelinating Schwann cells in peripheral nerves. The GFAP protein runs on gels at ~55 KDa protein, usually associated with lower Mw bands which are thought to be proteolytic fragments and alternate transcripts from the single gene.
Form:
Liquid
Concentration:
1 mg/mL
Isotype:
IgM
Recommend Usage:
Western Blot (1-2 ug/mL) The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction:
Store at 4°C. Do not freeze.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blotting analysis (reducing conditions) of GFAP in porcine brain lysate. Lane 1 : Immunostaining with GFAP monoclonal antibody, clone GF-02 (Cat # MAB3613). Lane 2 : Immunostaining with Isotype mouse IgM control.