DDDDK tag monoclonal antibody, clone F-tag-01
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More Files
- More Functions
Immunofluorescence
Confocal microscopy of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DDDDK epitope.
A : Fusion nuclear protein (red) stained with DDDDK monoclonal antibody, clone F-tag-01 (Cat # MAB3608) (detection by Goat anti-mouse IgG1 Alexa Fluor® 594).
B : Cell nuclei stained with DAPI (blue).
C : Merged figures - confirmation of nuclear localization of the fusion protein ; cell nuclei stained with DAPI (blue).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of DDDDK.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to DDDDK tag.
Sequence
DYKDDDDK
Host
Mouse
Specificity
This antibody recognizes DDDDK-tagged proteins in all species. The small size of this tag and its high hydrophilicity decrease the probability of interferencewith its expression, proteolytic maturation, antigenicity, localization and function. Recognizes fusion proteins in all species.
Form
Liquid
Isotype
IgG1
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunocytochemistry
Immunofluorescence
Confocal microscopy of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DDDDK epitope.
A : Fusion nuclear protein (red) stained with DDDDK monoclonal antibody, clone F-tag-01 (Cat # MAB3608) (detection by Goat anti-mouse IgG1 Alexa Fluor® 594).
B : Cell nuclei stained with DAPI (blue).
C : Merged figures - confirmation of nuclear localization of the fusion protein ; cell nuclei stained with DAPI (blue). -
Publication Reference
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Dazap2 modulates transcription driven by the Wnt effector TCF-4.
Lukas J, Mazna P, Valenta T, Doubravska L, Pospichalova V, Vojtechova M, Fafilek B, Ivanek R, Plachy J, Novak J, Korinek V.
Nucleic Acids Research 2009 Mar; 37(9):3007.
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HIC1 attenuates Wnt signaling by recruitment of TCF-4 and beta-catenin to the nuclear bodies.
Valenta T, Lukas J, Doubravska L, Fafilek B, Korinek V.
The EMBO Journal 2006 May; 25(11):2326.
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Dazap2 modulates transcription driven by the Wnt effector TCF-4.
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