Mouse monoclonal antibody raised against synthetic peptide of DDDDK.
A synthetic peptide (conjugated with KLH) corresponding to DDDDK tag.
This antibody recognizes DDDDK-tagged proteins in all species. The small size of this tag and its high hydrophilicity decrease the probability of interferencewith its expression, proteolytic maturation, antigenicity, localization and function. Recognizes fusion proteins in all species.
The optimal working dilution should be determined by the end user.
In PBS, pH 7.4 (0.09% sodium azide)
Store at 4°C. Do not freeze. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Confocal microscopy of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DDDDK epitope. A : Fusion nuclear protein (red) stained with DDDDK monoclonal antibody, clone F-tag-01 (Cat # MAB3608) (detection by Goat anti-mouse IgG1 Alexa Fluor® 594). B : Cell nuclei stained with DAPI (blue). C : Merged figures - confirmation of nuclear localization of the fusion protein ; cell nuclei stained with DAPI (blue).