AHNAK monoclonal antibody, clone EM-09
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More Files
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Immunofluorescence
Immunofluorescence staining of AHNAK in HeLa cell line using AHNAK monoclonal antibody, clone EM-09 (Cat # MAB3607 ; red). Cell nuclei stained with DAPI (blue).
Immunofluorescence
Immunofluorescence staining of AHNAK in human primary fibroblasts using AHNAK monoclonal antibody, clone EM-09 (Cat # MAB3607 ; green). Actin filaments were decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant AHNAK.
Immunogen
Recombinant protein corresponding to N-terminus human AHNAK.
Host
Mouse
Theoretical MW (kDa)
700
Reactivity
Human, Mouse
Specificity
This antibody reacts with AHNAK, a 700 KDa multi-functional adaptor proteinexpressed mainly in epithelial cell, various types of muscle cells and immune cells.
Form
Liquid
Isotype
IgG1
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunocytochemistry
Immunofluorescence
Immunofluorescence staining of AHNAK in HeLa cell line using AHNAK monoclonal antibody, clone EM-09 (Cat # MAB3607 ; red). Cell nuclei stained with DAPI (blue).Immunofluorescence
Immunofluorescence staining of AHNAK in human primary fibroblasts using AHNAK monoclonal antibody, clone EM-09 (Cat # MAB3607 ; green). Actin filaments were decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).Immunoprecipitation
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Gene Info — AHNAK
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Interactome
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Publication Reference
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No ligand binding in the GB2 subunit of the GABA(B) receptor is required for activation and allosteric interaction between the subunits.
Kniazeff J, Galvez T, Labesse G, Pin JP.
Journal of Neuroscience 2002 Sep; 22(17):7352.
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Function of GB1 and GB2 subunits in G protein coupling of GABA(B) receptors.
Margeta-Mitrovic M, Jan YN, Jan LY.
PNAS 2001 Nov; 98(25):14649.
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Gamma-aminobutyric acid type B receptors with specific heterodimer composition and postsynaptic actions in hippocampal neurons are targets of anticonvulsant gabapentin action.
Ng GY, Bertrand S, Sullivan R, Ethier N, Wang J, Yergey J, Belley M, Trimble L, Bateman K, Alder L, Smith A, McKernan R, Metters K, O'Neill GP, Lacaille JC, Hébert TE.
Molecular Pharmacology 2001 Jan; 59(1):144.
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No ligand binding in the GB2 subunit of the GABA(B) receptor is required for activation and allosteric interaction between the subunits.
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