ACR monoclonal antibody, clone ACR-2
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Specification
Product Description
Mouse monoclonal antibody raised against native ACR.
Immunogen
Native purified porcine ACR.
Host
Mouse
Theoretical MW (kDa)
55, 53, 45, 35
Reactivity
Pig
Specificity
This antibody reacts with various forms of porcine acrosin (55, 53, 45 and 35 KDa), a typical serine proteinase with trypsin-like specificity. Acrosin is stored in the acrosome of undamaged spermatozoa.
Form
Liquid
Isotype
IgG1
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunocytochemistry
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Gene Info — ACR
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Publication Reference
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Monoclonal antibodies to intra-acrosomal proteins inhibit gamete binding in vitro.
Peknicova J, Capkova J, Geussova G, Ivanova M, Mollova M.
Theriogenology 2001 Jul; 56(2):211.
Application:IF, Pig, Sperm.
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Protein-protein interactions controlling acrosin release and solubilization during the boar sperm acrosome reaction.
Moos J, Peknicova J, Tesarik J.
Biology of Reproduction 1993 Aug; 49(2):408.
Application:ELISA, IF, Pig, Sperm, Sperm extracted.
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Relationship between molecular conversions of acrosin and the progression of exocytosis in the calcium ionophore-induced acrosome reaction.
Moos J, Peknicova J, Tesarik J.
Biochimica et Biophysica Acta 1993 Apr; 1176(3):199.
Application:IF, WB-Ce, Pig, Sperm.
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Mechanism of maturation and nature of carbohydrate chains of boar sperm acrosin.
Moos J, Tesarik J, Leca G, Peknicova J.
FEBS Letters 1991 Dec; 294(1-2):27.
Application:WB, Pig, Pig sperm.
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Monoclonal antibodies against boar acrosomal antigens labelling undamaged acrosomes of spermatozoa in immunofluorescence test.
Peknicová J, Moos J.
Andrologia 1990 Sep; 22(5):427.
Application:IF, WB-Ce, Mouse, Sperm, Spermatozoa.
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Monoclonal antibodies to intra-acrosomal proteins inhibit gamete binding in vitro.
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