PRKACA monoclonal antibody, clone 6D2.1
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More Files
- More Functions
Dot Blot
Dot Blot analysis of GST and GST-fusion proteins using PRKACA monoclonal antibody, clone 6D2.1 (Cat # MAB3591) and GST monoclonal antibody, clone S-tag-05 (Cat # MAB3651).
The total amount of material spotted on the nitrocellulose membrane is 5 ng/spot.
Lane 1 : GST-Akt1.
Lane 2 : GST-Akt2.
Lane 3 : GST-Akt3.
Lane 4 : GST-PKAc alpha.
Lane 5 : GST-PKAc beta.
Lane 6 : GST-PKAc gamma.
Lane 7 : GST-MEK 1.
Lane 8 : GST.
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of PRKACA.
Immunogen
A synthetic peptide corresponding to human PRKACA.
Sequence
ESPAQNTAHLDQFERIK
Host
Mouse
Theoretical MW (kDa)
40
Reactivity
Human
Specificity
This antibody strongly reacts with human proteinkinase A catalytic (PRKACA) alpha subunit, and weakly with PKAc gamma subunit (both around 40 KDa). The recognized epitope of PKAc alpha is identical between man, sheep, pig, ox and dog.
Form
Liquid
Isotype
IgG
Recommend Usage
Western Blot (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Dot Blot
Dot Blot analysis of GST and GST-fusion proteins using PRKACA monoclonal antibody, clone 6D2.1 (Cat # MAB3591) and GST monoclonal antibody, clone S-tag-05 (Cat # MAB3651).
The total amount of material spotted on the nitrocellulose membrane is 5 ng/spot.
Lane 1 : GST-Akt1.
Lane 2 : GST-Akt2.
Lane 3 : GST-Akt3.
Lane 4 : GST-PKAc alpha.
Lane 5 : GST-PKAc beta.
Lane 6 : GST-PKAc gamma.
Lane 7 : GST-MEK 1.
Lane 8 : GST. -
Gene Info — PRKACA
Entrez GeneID
5566Gene Name
PRKACA
Gene Alias
MGC102831, MGC48865, PKACA
Gene Description
protein kinase, cAMP-dependent, catalytic, alpha
Omim ID
601639Gene Ontology
HyperlinkGene Summary
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
Other Designations
PKA C-alpha|cAMP-dependent protein kinase catalytic subunit alpha|cAMP-dependent protein kinase catalytic subunit alpha, isoform 1|protein kinase A catalytic subunit
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Interactome
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Pathway
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Publication Reference
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New faces of the familiar clathrin lattice.
Wilbur JD, Hwang PK, Brodsky FM.
Traffic (Copenhagen, Denmark) 2005 Apr; 6(4):346.
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Cargo- and compartment-selective endocytic scaffold proteins.
Szymkiewicz I, Shupliakov O, Dikic I.
The Biochemical Journal 2004 Oct; 383(Pt 1):1.
Application：WB, Human, Mammalian cells.
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Monoclonal antibody BF-06 against the heavy chain of clathrin.
Macurek L, Zikova M, Draberova E, Draber P.
Folia Biologica 2003 Dec; 49(6):238.
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New faces of the familiar clathrin lattice.
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