Mouse monoclonal antibody raised against synthetic peptide of PRKACA.
A synthetic peptide corresponding to human PRKACA.
Theoretical MW (kDa):
This antibody strongly reacts with human proteinkinase A catalytic (PRKACA) alpha subunit, and weakly with PKAc gamma subunit (both around 40 KDa). The recognized epitope of PKAc alpha is identical between man, sheep, pig, ox and dog.
Western Blot (0.5-1 ug/mL) The optimal working dilution should be determined by the end user.
In PBS, pH 7.4 (0.09% sodium azide)
Store at 4°C. Do not freeze. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Dot Blot analysis of GST and GST-fusion proteins using PRKACA monoclonal antibody, clone 6D2.1 (Cat # MAB3591) and GST monoclonal antibody, clone S-tag-05 (Cat # MAB3651). The total amount of material spotted on the nitrocellulose membrane is 5 ng/spot. Lane 1 : GST-Akt1. Lane 2 : GST-Akt2. Lane 3 : GST-Akt3. Lane 4 : GST-PKAc alpha. Lane 5 : GST-PKAc beta. Lane 6 : GST-PKAc gamma. Lane 7 : GST-MEK 1. Lane 8 : GST.
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
PKA C-alpha,cAMP-dependent protein kinase catalytic subunit alpha,cAMP-dependent protein kinase catalytic subunit alpha, isoform 1,protein kinase A catalytic subunit