Western blot analysis of Lane 1: Mouse Liver Tissue lysate, Lane 2: 293T cell lysate, Lane 3: EPHX1 Transfected 293T cell lysate.
Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of Hep3B cells. The cell was stained with EPHX1 monoclonal antibody, clone AT2E5 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Epoxide hydrolase is a critical biotransformation enzyme that converts epoxides from the degradation of aromatic compounds to trans-dihydrodiols which can be conjugated and excreted from the body. Epoxide hydrolase functions in both the activation and detoxification of epoxides. Mutations in this gene cause preeclampsia, epoxide hydrolase deficiency or increased epoxide hydrolase activity. Alternatively spliced transcript variants encoding the same protein have been found for this gene