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Last updated: 2019/12/15
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FKBP4 monoclonal antibody, clone AT4D3 

  • Catalog # : MAB3587
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against full length recombinant FKBP4.
  • Immunogen:
  • Recombinant protein corresponding to full length human FKBP4.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G affinity chromatography
  • Isotype:
  • IgG2b, kappa
  • Recommend Usage:
  • ELISA
    Flow Cytometry
    Immunocytochemistry/Immunofluorescence
    Immunohistochemistry (1:50-1:100)
    Western Blot (1:1000- 1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at 4°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Cell lysates of HeLa (40 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with FKBP4 monoclonal antibody, clone AT4D3 (1 : 1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of seminoma (germ cell tumor) tissue were incubated with MAB3587 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence staining of FKBP4 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and FKBP4 monoclonal antibody, clone AT4D3 (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
  • Enzyme-linked Immunoabsorbent Assay
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometry analysis of FKBP4 in HeLa cell line, staining at 2-5 ug for 1x106 cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Entrez GeneID:
  • 2288
  • Gene Name:
  • FKBP4
  • Gene Alias:
  • FKBP52,FKBP59,HBI,Hsp56,PPIase,p52
  • Gene Description:
  • FK506 binding protein 4, 59kDa
  • Gene Summary:
  • The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds to the immunosuppressants FK506 and rapamycin. It has high structural and functional similarity to FK506-binding protein 1A (FKBP1A), but unlike FKBP1A, this protein does not have immunosuppressant activity when complexed with FK506. It interacts with interferon regulatory factor-4 and plays an important role in immunoregulatory gene expression in B and T lymphocytes. This encoded protein is known to associate with phytanoyl-CoA alpha-hydroxylase. It can also associate with two heat shock proteins (hsp90 and hsp70) and thus may play a role in the intracellular trafficking of hetero-oligomeric forms of the steroid hormone receptors. This protein correlates strongly with adeno-associated virus type 2 vectors (AAV) resulting in a significant increase in AAV-mediated transgene expression in human cell lines. Thus this encoded protein is thought to have important implications for the optimal use of AAV vectors in human gene therapy. The human genome contains several non-transcribed pseudogenes similar to this gene. [provided by RefSeq
  • Other Designations:
  • 52 kD FK506 binding protein,FK506 binding protein 4 (59kD),FK506 binding protein 52,FK506-binding protein 4 (59kD),HSP binding immunophilin,T-cell FK506-binding protein, 59kD,p59 protein,peptidylprolyl cis-trans isomerase,rotamase
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