EPCAM monoclonal antibody, clone HEA125
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More Files
- More Functions
Immunofluorescence (Circulating Tumor Cell)
MCF-7 cells were stained with EPCAM-FITC labeled monoclonal antibody (Green). The cell nucleus were counterstained with DAPI (Blue).
Immunofluorescence
Immunofluorescence of monoclonal antibody to EpCAM on MCF-7 cell. [antibody concentration 10 ug/ml]
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Specification
Product Description
Mouse monoclonal antibody raised against native EPCAM.
Immunogen
Native purified EPCAM from living human colon carcinoma cell line HT-29.
Host
Mouse
Theoretical MW (kDa)
34
Reactivity
Human
Form
Liquid
Concentration
100 ug/mL
Isotype
IgG1
Recommend Usage
Immunohistochemistry (1:20)
Flow Cytometry(5 ul/100 ul cell suspension)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.3 (0.2% BSA, 0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
Flow Cytometry
Immunofluorescence (Circulating Tumor Cell)
MCF-7 cells were stained with EPCAM-FITC labeled monoclonal antibody (Green). The cell nucleus were counterstained with DAPI (Blue).Immunofluorescence
Immunofluorescence of monoclonal antibody to EpCAM on MCF-7 cell. [antibody concentration 10 ug/ml] -
Gene Info — EPCAM
Entrez GeneID
4072Gene Name
EPCAM
Gene Alias
17-1A, 323/A3, CD326, CO-17A, CO17-1A, EGP, EGP-2, EGP34, EGP40, ESA, Ep-CAM, GA733-2, HEA125, KS1/4, KSA, M4S1, MH99, MIC18, MK-1, MOC31, TACST-1, TACSTD1, TROP1, hEGP-2
Gene Description
epithelial cell adhesion molecule
Omim ID
185535Gene Ontology
HyperlinkGene Summary
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq
Other Designations
adenocarcinoma-associated antigen|carcinoma-associated antigen GA733-2|human epithelial glycoprotein-2|membrane component, chromosome 4, surface marker (35kD glycoprotein)|tumor-associated calcium signal transducer 1
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Interactome
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Disease
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Publication Reference
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Separation of tumor cells from a suspension of dissociated human colorectal carcinoma tissue by means of monoclonal antibody-coated magnetic beads.
Kemmner W, Moldenhauer G, Schlag P, Brossmer R.
Journal of Immunological Methods 1992 Mar; 147(2):197.
Application：Func, IF, IP, Human, HT-29 cells, Human colorectal tumor tissues.
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Epithelial glycoprotein is a member of a family of epithelial cell surface antigens homologous to nidogen, a matrix adhesion protein.
Simon B, Podolsky DK, Moldenhauer G, Isselbacher KJ, Gattoni-Celli S, Brand SJ.
PNAS 1990 Apr; 87(7):2755.
Application：IP, Human, COS, HT-29 cells.
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Immunohistochemical study of the expression of a Mr 34,000 human epithelium-specific surface glycoprotein in normal and malignant tissues.
Momburg F, Moldenhauer G, Hämmerling GJ, Möller P.
Cancer Research 1987 Jun; 47(11):2883.
Application：IHC-Fr, IHC-P, Human, Human malignant tissues, Human normal tissues.
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Separation of tumor cells from a suspension of dissociated human colorectal carcinoma tissue by means of monoclonal antibody-coated magnetic beads.
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