CASP2 monoclonal antibody, clone 11B4
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More Files
- More Functions
Western Blot (Cell lysate)
Detection of pro-CASP2 (p51) , an intermediate cleavage product of CASP2 (p32) and activated CASP2 (p19) in FDC-P1 cells (mouse IL-3 dependent promyelocytic line) during growth factor with drawal induced apoptosis. Activation of CASP2 is inhibited in the presence of the caspase inhibitor Z-VAD-FMK.
Western Blot
Western blot analysis of cell lysates from human and mouse cell lines with CASP2 monoclonal antibody, clone 11B4 (Cat # MAB3485). Clone 11B4 demonstrates its ability to recognize an approximately 51 kDa protein that corresponds to endogenous pro-CASP2. Hsp-70 was used as an equal loading control, and -/- CASP2 cells were used as a negative control.
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Specification
Product Description
Rat monoclonal antibody raised against partial recombinant CASP2.
Immunogen
Recombinant protein corresponding p19 subunit of human CASP2.
Host
Rat
Reactivity
Dog, Human, Monkey, Mouse, Rat
Specificity
Recognizes an epitope in the p19 subunit of human, mouse, rat, monkey and dog caspase-2. Detects bands of ~51 KDa (procaspase-2), ~32 KDa and ~18 KDa (cleavage products) by Western blot.
Form
Liquid
Isotype
IgG2a
Recommend Usage
Western Blot (2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Detection of pro-CASP2 (p51) , an intermediate cleavage product of CASP2 (p32) and activated CASP2 (p19) in FDC-P1 cells (mouse IL-3 dependent promyelocytic line) during growth factor with drawal induced apoptosis. Activation of CASP2 is inhibited in the presence of the caspase inhibitor Z-VAD-FMK.Western Blot
Western blot analysis of cell lysates from human and mouse cell lines with CASP2 monoclonal antibody, clone 11B4 (Cat # MAB3485). Clone 11B4 demonstrates its ability to recognize an approximately 51 kDa protein that corresponds to endogenous pro-CASP2. Hsp-70 was used as an equal loading control, and -/- CASP2 cells were used as a negative control.Immunoprecipitation
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Gene Info — CASP2
Entrez GeneID
835Gene Name
CASP2
Gene Alias
CASP-2, ICH-1L, ICH-1L/1S, ICH1, NEDD2
Gene Description
caspase 2, apoptosis-related cysteine peptidase
Omim ID
600639Gene Ontology
HyperlinkGene Summary
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The proteolytic cleavage of this protein is induced by a variety of apoptotic stimuli. Alternative splicing of this gene results in multiple transcript variants that encode different isoforms. [provided by RefSeq
Other Designations
ICH-1 protease|NEDD2 apoptosis regulatory|caspase 2|neural precursor cell expressed, developmentally down-regulated 2
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Interactome
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Disease
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