Panel A : Polyacrylamide gel electrophoresis (SDS-PAGE) and Coomassie brilliant blue staining of molecular weight standard (MW), pure GSTP1 protein and liver homogenate (5 ug protein) from normal rat liver (Control) or from liver tissue containing neoplastic lesions at different time points during hepatocarcinogenesis. Panel B : Western blot using GSTP1 monoclonal antibody, clone Usal-hGST-Pi-McAb-1 (Cat # MAB3452).
Immunohistochemistry of rat livers at 12, 20 or 32 weeks after initiation of liver carcinogensis. GSTP1-postive tissue was stained with GSTP1 monoclonal antibody, clone Usal-hGST-Pi-McAb-1 (Cat # MAB3452) in a 30 um thick rat liver slice. Original magnification 4X.
Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. [provided by RefSeq