Mouse monoclonal antibody raised against native PRKDC.
Native purified PRKDC from HeLa cells.
Chicken, Clawed frog, Hamster, Human, Mouse
Reacts with the nuclear catalytic subunit of DNA-dependent Protein Kinase (DNA-PKcs). Epitopes of the three clones have not been precisely mapped, but experimental data suggests that they are different.
IgG1; IgG2a; IgG1
Immunoprecipitation (2 ug/mg protein lysate) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-4 ug/mL) Western Blot (1-2 ug/mL) The optimal working dilution should be determined by the end user.
In 10 mM PBS, pH 7.4 (0.09% sodium azide)
Store at 4°C.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties.[supplied by OMIM
hyper-radiosensitivity of murine scid mutation, complementing 1