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Last updated: 2016/12/4
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Casp1 monoclonal antibody, clone 1H11

  • Catalog # : MAB3427
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  • Specification
  • Product Description:
  • Rat monoclonal antibody raised against synthetic peptide of Casp1.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 206-220 of mouse Casp1.
  • Sequence:
  • TALEMVKEVKEFAAC
  • Host:
  • Rat
  • Reactivity:
  • Mouse
  • Specificity:
  • Recognizes an epitope within the p20 fragment of mouse caspase-1. Detects a band of ~45 KDa by Western blot. Does not cross-react with mouse caspase-11 or -12.
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.02% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Casp1 monoclonal antibody, clone 1H11 (Cat # MAB3427) detect endogenous pro-Casp1 in spleen from WT mice, but not from spleen of Casp1 deficient mice. HSP-70 : loading control.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Casp1 monoclonal antibody, clone 1H11 (Cat # MAB3427) detect overexpressed Casp1 in 293T cells transiently transfected with a EE tagged inactive cysteine mutant of mouse Casp1 as a band of ~45 kDa.
  • ELISA
  • Flow Cytometry
  • Flow Cytometry
  • Casp1 monoclonal antibody, clone 1H11 (Cat # MAB3427) detect overexpressed Casp1 in 293T cells transiently transfected with an EE tagged inactive cysteine mutant of mouse Casp1 by flow cytometry.
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • Casp1
  • Gene Alias:
  • ICE,Il1bc
  • Gene Description:
  • caspase 1
  • Other Designations:
  • IL-1B converting enzyme,interleukin 1 beta-converting enzyme
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