Casp1 monoclonal antibody, clone 1H11
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More Files
- More Functions
Western Blot (Tissue lysate)
Casp1 monoclonal antibody, clone 1H11 (Cat # MAB3427) detect endogenous pro-Casp1 in spleen from WT mice, but not from spleen of Casp1 deficient mice. HSP-70 : loading control.
Western Blot (Transfected lysate)
Casp1 monoclonal antibody, clone 1H11 (Cat # MAB3427) detect overexpressed Casp1 in 293T cells transiently transfected with a EE tagged inactive cysteine mutant of mouse Casp1 as a band of ~45 kDa.
Flow Cytometry
Casp1 monoclonal antibody, clone 1H11 (Cat # MAB3427) detect overexpressed Casp1 in 293T cells transiently transfected with an EE tagged inactive cysteine mutant of mouse Casp1 by flow cytometry.
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Specification
Product Description
Rat monoclonal antibody raised against synthetic peptide of Casp1.
Immunogen
A synthetic peptide corresponding to amino acids 206-220 of mouse Casp1.
Sequence
TALEMVKEVKEFAAC
Host
Rat
Reactivity
Mouse
Specificity
Recognizes an epitope within the p20 fragment of mouse caspase-1. Detects a band of ~45 KDa by Western blot. Does not cross-react with mouse caspase-11 or -12.
Form
Liquid
Isotype
IgG1
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Casp1 monoclonal antibody, clone 1H11 (Cat # MAB3427) detect endogenous pro-Casp1 in spleen from WT mice, but not from spleen of Casp1 deficient mice. HSP-70 : loading control.Western Blot (Transfected lysate)
Casp1 monoclonal antibody, clone 1H11 (Cat # MAB3427) detect overexpressed Casp1 in 293T cells transiently transfected with a EE tagged inactive cysteine mutant of mouse Casp1 as a band of ~45 kDa.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Casp1 monoclonal antibody, clone 1H11 (Cat # MAB3427) detect overexpressed Casp1 in 293T cells transiently transfected with an EE tagged inactive cysteine mutant of mouse Casp1 by flow cytometry. -
Gene Info — Casp1
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