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Last updated: 2017/5/21

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V5 Tag monoclonal antibody, clone V5.C8

  • Catalog # : MAB3424
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic peptide of V5 Tag.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to amino acids 95-108 of simian virus 5 RNA polymerase alpha subunit.
  • Sequence:
  • GKPIPNPLLGLDST
  • Host:
  • Mouse
  • Specificity:
  • Anti-V5 Antibody detects (GKPIPNPLLGLDST) tag fusion protein.
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Western Blot (1:1000-1:10000)
    ELISA (For coating 1:100-1:500) (For primary 1:1000-1:30000)
    Immunocytochemistry (1:100-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.2 (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of 293 cells transfected with V5 tagged vector (Lane 2) and untransfected control (Lane 1). Using V5 Tag monoclonal antibody, clone V5.C8 (Cat # MAB3424).
  • Immunocytochemistry
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunocytochemistry
  • ELISA
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