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Last updated: 2017/9/24
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GFAP monoclonal antibody, clone SB61b

  • Catalog # : MAB3336
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant GFAP.
  • Immunogen:
  • Recombinant protein corresponding to human GFAP.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Specificity:
  • Reacts with human GFAP (~ 50 KDa).
  • Form:
  • Liquid
  • Isotype:
  • IgG2b
  • Recommend Usage:
  • Western Blot (2 ug/mL)
    Immunohistochemistry (5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 100 mM BBS, pH 8.0
  • Storage Instruction:
  • Store at 4°C.
  • Publication Reference
  • Applications
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Frozen sections)
  • Immunofluorescence
  • Immunofluorescence
  • The CCF-STTG1 cell line was fixed with 5% Acetic acid Methanol solution for 30 minutes followed by 5% Bovine serum PBS blocking , cells are permeabilized by 0.1% Triton X PBS for 15 mines before and stained with GFAP monoclonal antibody, clone SB61b (Cat # MAB3336) and then cells were fixed. (The cell washing buffer was 0.15% tween 20 in PBS.) Finally the slide was mounted with Fluormount-G contained DAPI.
  • Application Image
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Frozen sections)
  • Gene Information
  • Entrez GeneID:
  • 2670
  • Gene Name:
  • GFAP
  • Gene Alias:
  • FLJ45472
  • Gene Description:
  • glial fibrillary acidic protein
  • Gene Summary:
  • This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq
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