Il5 monoclonal antibody, clone TRFK4
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Specification
Product Description
Rat monoclonal antibody raised against native Il-5.
Immunogen
Native semi-purified mouse T cell clone supernatant.
Host
Rat
Reactivity
Mouse
Specificity
Mouse Interleukin-5 (IL-5). This is a neutralizing antibody.
Form
Liquid
Isotype
IgG2a
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In 100 mM BBS, pH 8.2
Storage Instruction
Store at 4°C.
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Applications
Sandwich ELISA
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Gene Info — Il5
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Publication Reference
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Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining.
B Sander, I Höidén, U Andersson, E Möller, J S Abrams.
Journal of Immunological Methods 1993 Dec; 166(2):201.
Application:EIA, IF, Mouse, T cells, T cells culture supernatants.
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Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE.
Immunological Reviews 2006 Jun; 127:5.
Application:Blocking, ELISA, Mouse, Supernatants from cells cultured and stimulated ex vivo with different agents and biological fluids like sera/plasma, bronehoalveolar lavage, ascites, urines, cerebrospinal fluids, saliva, and sputa. .
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The characterization of four monoclonal antibodies specific for mouse IL-5 and development of mouse and human IL-5 enzyme-linked immunosorbent.
Schumacher JH, O'Garra A, Shrader B, van Kimmenade A, Bond MW, Mosmann TR, Coffman RL.
The Journal of Immunology 1988 Sep; 141(5):1576.
Application:ELISA, Recombinant protein.
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Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining.
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