H2-D2 monoclonal antibody, clone 28-14-8
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More Files
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Flow Cytometry
Splenocytes from DBA1/J mice were stained with H2-D2 monoclonal antibody, clone 28-14-8 (Cat # MAB3258)-FITC. Small lymphocytes were then gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
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Specification
Product Description
Mouse monoclonal antibody raised against H2-D2.
Immunogen
Native from C3H.SW mouse skin graft and splenocytes.
Host
Mouse
Reactivity
Mouse
Specificity
An epitope in the alpha3 domain of H-2Db.
Form
Liquid
Isotype
IgG2a
Recommend Usage
Flow Cytometry (1 ug/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In 100 mM BBS, pH 8.2
Storage Instruction
Store at 4°C.
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Applications
Immunohistochemistry (Frozen sections)
Flow Cytometry
Splenocytes from DBA1/J mice were stained with H2-D2 monoclonal antibody, clone 28-14-8 (Cat # MAB3258)-FITC. Small lymphocytes were then gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA). -
Publication Reference
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Cloning and functional characteristics of murine large granular lymphocyte-1: a member of the Ly-49 gene family (Ly-49G2).
Mason LH, Ortaldo JR, Young HA, Kumar V, Bennett M, Anderson SK.
The Journal of Experimental Medicine 1995 Aug; 182(2):293.
Application：Flow Cyt, Mouse, Mouse natural killer cells.
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Fibroblasts as efficient antigen-presenting cells in lymphoid organs.
Kundig TM, Bachmann MF, DiPaolo C, Simard JJ, Battegay M, Lother H, Gessner A, Kuhlcke K, Ohashi PS, Hengartner H, et al..
Science 1995 Jun; 268(5215):1343.
Application：Func, Mouse, L929 cells.
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Beta 2-microglobulin deficient mice lack CD4-8+ cytolytic T cells.
Zijlstra M, Bix M, Simister NE, Loring JM, Raulet DH, Jaenisch R.
Nature 1990 Apr; 344(6268):742.
Application：Flow Cyt, Mouse, Mouse embryonic fibroblasts.
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Cloning and functional characteristics of murine large granular lymphocyte-1: a member of the Ly-49 gene family (Ly-49G2).
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