Ly6a monoclonal antibody, clone D7
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More Files
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Flow Cytometry
BALB/c spleen cells were activated by incubation for 48 hours with concanavalin-A. After harvesting, the cells were stained with Ly6a monoclonal antibody, clone D7 (Cat # MAB3239)-FITC, and activated cells were gated and analyzed on a FACScan™ flow cytometer.
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Specification
Product Description
Rat monoclonal antibody raised against Ly6a.
Immunogen
IL-2-dependent mouse T-cell line CTL-L.
Host
Rat
Reactivity
Mouse
Specificity
Ly-6A.2 and Ly-6E.1, allelic members of the Ly-6 multigene family; Mr 18 KDa.
Form
Liquid
Isotype
IgG2a
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In 100 mM BBS, pH 8.2
Storage Instruction
Store at 4°C.
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Applications
Western Blot
Immunohistochemistry (Frozen sections)
Immunoprecipitation
Flow Cytometry
BALB/c spleen cells were activated by incubation for 48 hours with concanavalin-A. After harvesting, the cells were stained with Ly6a monoclonal antibody, clone D7 (Cat # MAB3239)-FITC, and activated cells were gated and analyzed on a FACScan™ flow cytometer. -
Gene Info — Ly6a
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Publication Reference
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In vitro expansion of hematopoietic progenitors and maintenance of stem cells: comparison between FLT3/FLK-2 ligand and KIT ligand.
Yonemura Y, Ku H, Lyman SD, Ogawa M.
Blood 1997 Mar; 89(6):1915.
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In vitro differentiation of murine Sca-1+Lin- cells into myeloid, B cell and T cell lineages.
Ito M, Anan K, Misawa M, Kai S, Hara H.
Stem Cells 1996 Jul; 14(4):412.
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Transplantable NK cell progenitors in murine bone marrow.
Moore T, Bennett M, Kumar V.
Journal of Immunology 1995 Feb; 154(4):1653.
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In vitro expansion of hematopoietic progenitors and maintenance of stem cells: comparison between FLT3/FLK-2 ligand and KIT ligand.
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