Cd86 monoclonal antibody, clone GL1
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More Files
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Flow Cytometry
BALB/c spleen cells were activated by incubation for 72 hours with LPS. The cells were then harvested and stained with Cd86 monoclonal antibody, clone GL1 (Cat # MAB3238)-FITC. A wide gate was set to include small, medium and large cells, following which the cells were analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
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Specification
Product Description
Rat monoclonal antibody raised against Cd86.
Immunogen
Native Cd86 from LPS-activated mouse B cells.
Host
Rat
Reactivity
Mouse
Specificity
Mouse CD86, the B7-2 co-stimulatory molecule and a ligand for CD28 and CD152/CTLA-4.
Form
Liquid
Isotype
IgG2a
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In 100 mM BBS, pH 8.2
Storage Instruction
Store at 4°C.
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Applications
Immunohistochemistry (Frozen sections)
Immunoprecipitation
Flow Cytometry
BALB/c spleen cells were activated by incubation for 72 hours with LPS. The cells were then harvested and stained with Cd86 monoclonal antibody, clone GL1 (Cat # MAB3238)-FITC. A wide gate was set to include small, medium and large cells, following which the cells were analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA). -
Gene Info — Cd86
Entrez GeneID
12524Gene Name
Cd86
Gene Alias
B7, B7-2, B7.2, B70, CLS1, Cd28l2, ETC-1, Ly-58, Ly58, MB7, MB7-2, TS/A-2
Gene Description
CD86 antigen
Gene Ontology
HyperlinkOther Designations
OTTMUSP00000015940|T-lymphocyte activation antigen CD86|activation B7-2 antigen|early T cell costimulatory molecule-1
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Publication Reference
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Cellular interaction in germinal centers. Roles of CD40 ligand and B7-2 in established germinal centers.
Han S, Hathcock K, Zheng B, Kepler TB, Hodes R, Kelsoe G.
Journal of Immunology 1995 Jul; 155(2):556.
Application：Func, IHC-Fr, Mouse, Mouse spleen.
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Distinct roles for the costimulatory ligands B7-1 and B7-2 in T helper cell differentiation?
Thompson CB.
Cell 1995 Jun; 81(7):979.
Application：Flow Cyt, Func, WB-Ce, WB-Tr, Human, Mouse, B cells, Dendritic cells, Mammalian cells, Monocytes, T cells.
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Comparative analysis of B7-1 and B7-2 costimulatory ligands: expression and function.
Hathcock KS, Laszlo G, Pucillo C, Linsley P, Hodes RJ.
The Journal of Experimental Medicine 1994 Aug; 180(2):631.
Application：Flow Cyt, Func, Mouse, Mouse B cells, CHO cells, Macrophages, T cells.
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Cellular interaction in germinal centers. Roles of CD40 ligand and B7-2 in established germinal centers.
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