Cd69 monoclonal antibody, clone H1.2F3
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More Files
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Flow Cytometry
Fresh BALB/c spleen cells were activated by incubation for 20 hours with PMA + ionomycin, then stained with either hamster IgG-FITC (as an isotype control) or Cd69 monoclonal antibody, clone H1.2F3 (Cat # MAB3236)-FITC. Large, activated cells were then gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
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Specification
Product Description
Hamster monoclonal antibody raised against Cd69.
Immunogen
Native Cd69 from Y245 mouse dendritic epidermal T cell line.
Host
Hamster
Reactivity
Mouse
Specificity
Mouse CD69/Very Early Activation Antigen (VEA), Mr 85 KDa (unreduced).
Form
Liquid
Isotype
IgG
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In 100 mM BBS, pH 8.2
Storage Instruction
Store at 4°C.
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Applications
Immunohistochemistry (Frozen sections)
Immunoprecipitation
Flow Cytometry
Fresh BALB/c spleen cells were activated by incubation for 20 hours with PMA + ionomycin, then stained with either hamster IgG-FITC (as an isotype control) or Cd69 monoclonal antibody, clone H1.2F3 (Cat # MAB3236)-FITC. Large, activated cells were then gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA). -
Gene Info — Cd69
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Publication Reference
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The activation antigen CD69.
Ziegler SF, Ramsdell F, Alderson MR.
Stem Cells 1994 Sep; 12(5):456.
Application：Flow Cyt, Human, Mouse, B cells, Myeloid cells, Natural killer cells, Platelets, T cells, Thymocytes.
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The mouse CD69 gene. Structure, expression, and mapping to the NK gene complex.
Ziegler SF, Levin SD, Johnson L, Copeland NG, Gilbert DJ, Jenkins NA, Baker E, Sutherland GR, Feldhaus AL, Ramsdell F.
Journal of Immunology 1994 Feb; 152(3):1228.
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Regulation of RAG-1 and CD69 expression in the thymus during positive and negative selection.
Brandle D, Muller S, Muller C, Hengartner H, Pircher H.
European Journal of Immunology 1994 Jan; 24(1):145.
Application：Flow Cyt, Mouse, Mouse thymocytes.
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The activation antigen CD69.
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