Ptprc exon B-dependent form monoclonal antibody, clone C363.16A
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More Files
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Flow Cytometry
BALB/c splenocytes were double-stained with Ptprc exon B-dependent form monoclonal antibody, clone C363.16A (Cat # MAB3233)-R-phycoerythrinand rat anti-mouse CD8a-FITC. Small lymphocytes were then gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
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Specification
Product Description
Rat monoclonal antibody raised against Ptprc exon B-dependent form.
Immunogen
Cloned mouse Th2 cell lines.
Host
Rat
Reactivity
Mouse
Specificity
An exon B-dependent epitope of CD45 glycoprotein.
Form
Liquid
Isotype
IgG2a
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In 100 mM BBS, pH 8.2
Storage Instruction
Store at 4°C.
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Applications
Immunoprecipitation
Flow Cytometry
BALB/c splenocytes were double-stained with Ptprc exon B-dependent form monoclonal antibody, clone C363.16A (Cat # MAB3233)-R-phycoerythrinand rat anti-mouse CD8a-FITC. Small lymphocytes were then gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA). -
Gene Info — Ptprc
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Interactome
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Publication Reference
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Expression of variable exon A-, B-, and C-specific CD45 determinants on peripheral and thymic T cell populations.
Hathcock KS, Laszlo G, Dickler HB, Sharrow SO, Johnson P, Trowbridge IS, Hodes RJ.
Journal of Immunology 1992 Jan; 148(1):19.
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A monoclonal antibody to murine CD45R distinguishes CD4 T cell populations that produce different cytokines.
Bottomly K, Luqman M, Greenbaum L, Carding S, West J, Pasqualini T, Murphy DB.
European Journal of Immunology 1989 Apr; 19(4):617.
Application：IP, Mouse, Mouse CD4+ T cells, thymocytes, spleen cells.
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Identification of the alternatively spliced exons of murine CD45 (T200) required for reactivity with B220 and other T200-restricted antibodies.
Johnson P, Greenbaum L, Bottomly K, Trowbridge IS.
The Journal of Experimental Medicine 1989 Mar; 169(3):1179.
Application：Flow Cyt, Mouse, Ψ2 cells .
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Expression of variable exon A-, B-, and C-specific CD45 determinants on peripheral and thymic T cell populations.
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