Pecam1 monoclonal antibody, clone 390
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Flow Cytometry
BALB/c splenocytes were first incubated with purified Pecam1 monoclonal antibody, clone 390 (Cat # MAB3230) and then with goat anti-rat Ig-FITC. Small lymphocytes were gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
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Specification
Product Description
Rat monoclonal antibody raised against native Pecam1.
Immunogen
Native Pecam1 from mouse leukocyte cell line 32D.
Host
Rat
Reactivity
Mouse
Specificity
Mouse CD31/PECAM-1, Mr 130-140 KDa.
Form
Liquid
Isotype
IgG2a
Recommend Usage
Flow Cytometry (3 ug/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In 100 mM BBS, pH 8.2
Storage Instruction
Store at 4°C.
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Applications
Immunohistochemistry (Frozen sections)
Immunoprecipitation
Flow Cytometry
BALB/c splenocytes were first incubated with purified Pecam1 monoclonal antibody, clone 390 (Cat # MAB3230) and then with goat anti-rat Ig-FITC. Small lymphocytes were gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA). -
Gene Info — Pecam1
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Interactome
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Publication Reference
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Platelet endothelial cell adhesion molecule (CD31).
DeLisser HM, Newman PJ, Albelda SM.
Current Topics in Microbiology and Immunology 1993 Jan; 184:37.
Application：Flow Cyt, Func, WB-Tr, Human, Human endothelial cells, Human granulocytes, Human monocytes, Human platelets.
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Platelet endothelial cell adhesion molecule (CD31).
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